Genomic study of hemagglutinins of swine influenza (H1N1) viruses associated with acute and chronic respiratory diseases in pigs

The nucleotide sequences of HA1 domain of hemagglutinin of clinical H1N1 influenza viruses, isolated during recent outbreaks of respiratory problems in pig farms of Quebec, was determined. The viruses A/Sw/Quebec/3291/90 (SwQc3291) and A/Sw/Quebec/1747/90 (SwQc1747), associated with chronic respirat...

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Bibliographic Details
Published in:Archives of virology 1997-01, Vol.142 (2), p.401-412
Main Authors: ARORA, D. J. S, N'DIAYE, M, DEA, S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, Viral - genetics
Biological and medical sciences
Epidemiology
Fundamental and applied biological sciences. Psychology
Hemagglutinins - genetics
Hemagglutinins - immunology
Influenza A virus - genetics
Influenza A Virus, H1N1 Subtype
Microbiology
Molecular Sequence Data
Orthomyxoviridae Infections - veterinary
Orthomyxoviridae Infections - virology
Respiratory diseases
Sequence Alignment
Swine
Swine Diseases - virology
Swine flu
Virology
Viruses
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Summary:The nucleotide sequences of HA1 domain of hemagglutinin of clinical H1N1 influenza viruses, isolated during recent outbreaks of respiratory problems in pig farms of Quebec, was determined. The viruses A/Sw/Quebec/3291/90 (SwQc3291) and A/Sw/Quebec/1747/90 (SwQc1747), associated with chronic respiratory disease, showed close similarity for their deduced aa sequences. When compared with the published data of A/Sw/Quebec/5393/91 (SwQc91), the variations observed included Cb and Ca antigenic sites in SwQc3291 and Sb and Ca sites in SwQc1747 isolates. These variants were antigenically related to SwQc91 virus associated with chronic respiratory disease, but differed from the more classical A/Sw/Quebec/192/81 (SwQc81) strain. In contrast, A/Sw/Quebec/1192/86 (SwQc1192) isolate, associated with acute respiratory influenza, showed maximum number of differences including Ca, Cb, Sa and Sb antigenic sites. The latter, as well as the SwQc81 strain, were antigenically distinct from SwQc91 virus on the basis of its cross-reactivity to MAbs directed against the HA glycoprotein. Estimation of genetic distances and phylogenic tree analysis showed that SwQc1747 and SwQc3291 were closely related, but these viruses along with SwQc1192 were considerably divergent from SwQc91 virus.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050086