A tandem array of 5S ribosomal RNA genes in Pythium irregulare

The 5S ribosomal RNA genes of the oomycete Pythium irregulare exist in tandem arrays unlinked to the rDNA repeat unit. A clone with a 9.2-kb insert containing an array of 5S genes was identified in a lambda genomic library and was characterized by restriction mapping and partial sequencing. The arra...

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Bibliographic Details
Published in:Gene 1997-02, Vol.186 (2), p.155-159
Main Authors: Belkhiri, Abbes, Intengan, Hope, Klassen, Glen R
Format: Article
Language:English
Subjects:
5S rRNA spacer
Base Sequence
Cloning, Molecular
DNA, Ribosomal - genetics
genes
Genes, Fungal
Genomic Library
molecular conformation
Molecular Sequence Data
Nucleic Acid Conformation
nucleotide sequences
Oomycetes
Polymerase Chain Reaction
protein secondary structure
Pseudogene
Pseudogenes
Pythium - genetics
Pythium irregulare
rDNA
Restriction Mapping
ribosomal DNA
ribosomal RNA
RNA, Fungal - genetics
RNA, Ribosomal, 5S - chemistry
RNA, Ribosomal, 5S - genetics
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Summary:The 5S ribosomal RNA genes of the oomycete Pythium irregulare exist in tandem arrays unlinked to the rDNA repeat unit. A clone with a 9.2-kb insert containing an array of 5S genes was identified in a lambda genomic library and was characterized by restriction mapping and partial sequencing. The array consisted of 9 apparently identical 5S genes and their spacers in tandem, followed by a diverged 5S-like sequence that is likely to be a pseudogene. This gene arrangement, although almost universal in plants and animals, is rare in fungi and protists.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(96)00662-2