Postvaccinal immune response to regimens of Newcastle disease vaccination by filter paper sampling technique

Seven hundred and ten blood samples were collected at random from commercial layers in Tamil Nadu on Whatman filter paper No. 1 instead of the conventional method of serum collection. The birds were subjected to different Newcastle disease (ND) vaccination schedules and samples were collected to stu...

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Bibliographic Details
Published in:Tropical animal health and production 1997-02, Vol.29 (1), p.20-24
Main Authors: Roy, P, Nachimuthu, K, Koteeswaran, A, Albert, A, Venugopalan, A T
Format: Article
Language:English
Subjects:
Agglutination Tests - methods
Agglutination Tests - veterinary
Animals
Antibodies
Antibodies, Viral - blood
Antibodies, Viral - metabolism
Blood Specimen Collection - methods
Blood Specimen Collection - veterinary
Chickens
Female
Filter paper
Hemagglutination inhibition
Immune response
Immune system
Immunization Schedule
India
Newcastle disease
Newcastle Disease - blood
Newcastle Disease - immunology
Newcastle Disease - prevention & control
Newcastle disease virus - immunology
Sampling methods
Vaccination
Vaccination - veterinary
Viral Vaccines - administration & dosage
Viral Vaccines - immunology
Viral Vaccines - standards
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Summary:Seven hundred and ten blood samples were collected at random from commercial layers in Tamil Nadu on Whatman filter paper No. 1 instead of the conventional method of serum collection. The birds were subjected to different Newcastle disease (ND) vaccination schedules and samples were collected to study the vaccinal response to ND at field level. Eluates were obtained from sample areas of filter paper using Brij-35 solution [detergent] and subjected to the micro haemagglutination inhibition (HI) test for ND antibodies. The HI titre ranged from less than 2(4) to 2(9). The possible causes of poor immune response to ND vaccinations are discussed.
ISSN:0049-4747
1573-7438
DOI:10.1007/BF02632339