Hyposmolar medium and ethanol in isosmotic solution induce the release of thyrotropin-releasing hormone (TRH) by isolated rat pancreatic islets

Cell swelling induced by hypotonic medium or small isotonic permeant molecules results in an immediate secretory response in various types of cells. We have expanded exploration of this phenomenon by examining the effect of either isotonic ethanol or hyposmotic medium on the release of TRH by freshl...

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Bibliographic Details
Published in:Life sciences (1973) 1997, Vol.60 (11), p.865-872
Main Authors: Benický, J., Greer, M.A., Štrbák, V.
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Calcium - pharmacology
Cell Size - drug effects
cell swelling
Cells, Cultured
Central Nervous System Depressants - pharmacology
ethanol
Ethanol - pharmacology
hyposmolarity
Hypotonic Solutions
Islets of Langerhans - cytology
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Isotonic Solutions
Kinetics
Male
pancreatic islets
Rats
Rats, Wistar
Stimulation, Chemical
thyrotropin-releasing hormone
Thyrotropin-Releasing Hormone - metabolism
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Summary:Cell swelling induced by hypotonic medium or small isotonic permeant molecules results in an immediate secretory response in various types of cells. We have expanded exploration of this phenomenon by examining the effect of either isotonic ethanol or hyposmotic medium on the release of TRH by freshly isolated islets of Langerhans in static incubation and perifusion. Ethanol (40, 80 or 160 mM in isotonic solution) dose-dependently evoked the release of TRH by statically incubated islets. The dynamics of TRH release induced by 80 mM isotonic ethanol or 30% hypotonic medium were similar to those induced by 50 mM KCl, with the highest secretion rate during the first 5 min of incubation irrespective of the duration of stimulation. Ca 2+ depletion of the incubation medium abolished the response to 50 mM KCl but did not diminish the response to 80 mM isotonic ethanol. We conclude that osmotic stimuli known to induce cell swelling also induce release of TRH by isolated pancreatic islets.
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(97)00015-5