Serratia liquefaciens as a new host superior for overproduction and purification using the N-acetylneuraminate lyase gene of Escherichia coli

Serratia liquefaciens was screened as a host strain for effective gene expression and easy purification of the target protein. A model gene, N-acetylneuraminate lyase gene (nanA), fused with the promoter region of Escherichia coli lac operon successfully overproduced the protein independently from t...

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Bibliographic Details
Published in:Analytical biochemistry 1997-03, Vol.246 (2), p.171-175
Main Authors: Yamamoto, K, Kawakami, B, Kawamura, Y, Kawai, K
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - isolation & purification
Bacterial Proteins - metabolism
Escherichia coli - enzymology
Escherichia coli - genetics
Gene Expression
Genetic Vectors
Heating
Hydrogen-Ion Concentration
Oxo-Acid-Lyases - genetics
Oxo-Acid-Lyases - isolation & purification
Oxo-Acid-Lyases - metabolism
Plasmids
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - isolation & purification
Recombinant Fusion Proteins - metabolism
Serratia - metabolism
Temperature
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Summary:Serratia liquefaciens was screened as a host strain for effective gene expression and easy purification of the target protein. A model gene, N-acetylneuraminate lyase gene (nanA), fused with the promoter region of Escherichia coli lac operon successfully overproduced the protein independently from the inducer. Since S. liquefaciens grew at lower temperature than E. coli and its proteins were more heat sensitive than those of E. coli, simple incubation at 60 degrees C could inactivate most enzymes but the nanA protein. Subsequent column works for purification, then, became simple and rapid.
ISSN:0003-2697
DOI:10.1006/abio.1997.2001