FISH landmarks for Aedes aegypti chromosomes

Aedes aegypti metaphase chromosome landmarks have been developed so that each chromosome of the haploid genome can be unambiguously identified and oriented by fluorescence in situ hybridization (FISH) and digital imaging microscopy. The FISH tags were derived from three cosmids that contain repetiti...

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Bibliographic Details
Published in:Insect molecular biology 1997-05, Vol.6 (2), p.197-202
Main Authors: Brown, S.E, Knudson, D.L
Format: Article
Language:English
Subjects:
Aedes
Aedes - genetics
Aedes aegypti
aegypti
Animals
Base Sequence
Cell Line
chromosome
chromosome mapping
chromosome tags
Chromosomes
cosmids
Culicidae
DNA
FISH
fluorescence
fluorescence in situ hybridization
Freshwater
genbank/u65373
genbank/u65374
genbank/u65375
In Situ Hybridization, Fluorescence
Keywords
Molecular Sequence Data
nucleic acid hybridization
nucleotide sequences
physical
plasmids
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Description
Summary:Aedes aegypti metaphase chromosome landmarks have been developed so that each chromosome of the haploid genome can be unambiguously identified and oriented by fluorescence in situ hybridization (FISH) and digital imaging microscopy. The FISH tags were derived from three cosmids that contain repetitive Ae. aegypti sequences and their unique FISH tagging characteristics are demonstrated. The sequence of the three chromosomal tags revealed that the chromosome 1 tag is an 18S fragment from the ribosomal cistron, and the other two chromosomal tags are repeats found in Ae. aegypti with no apparent similarity to known sequence. A single plasmid that contains the three chromosome tag sequences has been constructed to simplify future FISH physical mapping.
ISSN:0962-1075
1365-2583
DOI:10.1111/j.1365-2583.1997.tb00088.x