The Arabidopsis thaliana cDNA sequencing projects

Nearly 30 000 Arabidopsis thaliana EST (Expressed Sequence Tags) have been produced by a French and an American consortium. Despite redundancy these sequences tag about half the expected Arabidopsis genes. Approximately 40% of the non-redundant EST can be assigned a putative function by simple homol...

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Bibliographic Details
Published in:FEBS Letters 1997-03, Vol.405 (2), p.129-132
Main Authors: Delseny, Michel, Cooke, Richard, Raynal, Monique, Grellet, Françoise
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
cDNA
Crucifer
DNA, Complementary - genetics
Expressed sequence tag
Gene Expression
Gene function
Genes, Plant
Genome sequencing
Genome, Plant
Sequence Analysis, DNA
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Summary:Nearly 30 000 Arabidopsis thaliana EST (Expressed Sequence Tags) have been produced by a French and an American consortium. Despite redundancy these sequences tag about half the expected Arabidopsis genes. Approximately 40% of the non-redundant EST can be assigned a putative function by simple homology search. This programme allowed the identification of a large number of genes which would have been very difficult to isolate by other classical techniques. It considerably stimulated many areas of plant biology by the rapid discovery a large number of genes, by revealing multigene families and by allowing the analysis of differential expression of the different members. Finally this programme facilitated construction of physical maps of the chromosomes and opened the way for complete sequencing of the Arabidopsis genome and comparative mapping of the major plant crops. © 1997 Federation of European Biochemical Societies.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00184-1