Effects of Human Interleukin-3 on Granulocytic Colony-Forming Cells in Human Bone Marrow

• Published in: Blood 1989-04, Vol.73 (5), p.1157-1160
• Main Authors: Bot, Fredrik J., Eijk, Loes van, Schipper, Pauline, Lowenberg, Bob
• Format: Article
• Language: English
• Subjects: Adult; Analysis of the immune response. Humoral and cellular immunity; Antigens, CD34; Antigens, Differentiation; Biological and medical sciences; Bone Marrow - drug effects; Cell Separation; Cell Survival - drug effects; Colony-Forming Units Assay; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Granulocytes - classification; Granulocytes - drug effects; Granulocytes - physiology; Hematopoietic Stem Cells - classification; Hematopoietic Stem Cells - drug effects; Humans; Immunobiology; Interleukin-3 - pharmacology; Leukocyte Count - drug effects; Lymphokines, interleukins ( function, expression); Monocytes; Phenotype; Regulatory factors and their cellular receptors
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V73.5.1157.1157

Human multilineage colony-stimulating factor (multi-CSF)/ interleukin-3 (IL-3) induces colony formation from CFU-GEMM, BFU-E, and CFU-Eo when applied to in vitro cultures of highly enriched hematopoietic progenitor cells. No granulocytic colonies are formed in response to IL-3. However, with appropriate assays, we demonstrate that IL-3 increases the size of G-CSF-induced granulocytic colonies; these colonies contain greater proportions of immature cells as compared with colonies stimulated by G-CSF alone. Furthermore, IL-3 promotes the survival of CFU-G in vitro, whereas in cultures not supplemented with IL-3, CFU-G extinguish within seven days. We conclude that IL-3, although it does not stimulate granulocytic colony formation by itself, regulates the survival and proliferative rate of granulocytic progenitors.