Estrogen Treatment Increases Phospholipid Transfer Activities in Chicken Liver

• Published in: The Journal of biological chemistry 1989-04, Vol.264 (12), p.6612-6614
• Main Authors: Rusiñol, A E, Bloj, B
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Binding and carrier proteins; Biological and medical sciences; Carrier Proteins - analysis; Carrier Proteins - metabolism; Chickens - metabolism; Chromatography, Gel; estradiol; Estradiol - pharmacology; Fundamental and applied biological sciences. Psychology; Liver - metabolism; Membrane Proteins; Phosphatidylcholines - metabolism; Phosphatidylethanolamines - metabolism; Phosphatidylinositols - metabolism; Phospholipid Transfer Proteins; phospholipids; Phospholipids - metabolism; Proteins; Time Factors
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)83471-8

The effect of subcutaneous β-estradiol injection on liver phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol transfer activity of immature chicken has been determined. The estrogen administration significantly enhanced the transfer activity of both phosphatidylcholine (100%), phosphatidylethanolamine (160%), and phosphatidylinositol (150%). In vivo experiments revealed that the hormone-induced changes in liver lipid transfer activity were sensitive to a protein synthesis inhibitor, cycloheximide. A partial characterization of liver protein transfer on Sephacryl S-200 showed that multiple transfer proteins are involved in the β-estradiol effect. This is the first time that hormonal modulation of phospholipid transfer activities is described, and the results suggest that the hepatic phospholipid transfer activities might be involved in the biosynthesis of plasma lipoproteins in vivo.