The prostate-specific antigen gene and the human glandular kallikrein-1 gene are tandemly located on chromosome 19

Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and...

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Bibliographic Details
Published in:FEBS letters 1989-04, Vol.247 (1), p.123-126
Main Authors: Riegman, P.H.J., Vlietstra, R.J., Klaassen, P., van der Korput, J.A.G.M., van Kessel, A.Geurts, Romijn, J.C., Trapman, J.
Format: Article
Language:English
Subjects:
Animals
Antigens, Neoplasm - genetics
Chromosome 19
Chromosome Mapping
Chromosomes, Human, Pair 19
Cloning, Molecular
Cricetinae
Deoxyribonuclease BamHI
Deoxyribonuclease HindIII
DNA Probes
Glandular kallikrein
Human
Humans
Hybrid Cells
Kallikrein
Kallikreins - genetics
Kidney - enzymology
Male
Nucleic Acid Hybridization
Pancreas - enzymology
Prostate
Prostate-Specific Antigen
Tissue Kallikreins
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Summary:Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandular kallikrein-1 gene overlap and span a region of about 36 kb. The two genes are aligned in a head to tail orientation at a mutual distance of 12 kb. Southern blot analysis of DNA from a panel of human-hamster hybrid cells with specific probes revealed the genes to be situated on chromosome 19. Assuming that the pancreas/kidney kallikrein gene is located in the same cluster, the distance to the prostate-specific antigen gene and the human glandular kallikrein gene must be at least 15 kb.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)81253-0