Sulfatides inhibit binding of Helicobacter pylori to the gastric cancer Kato III cell line

Helicobacter pylori adhere to Kato III and Hela S3 cells in monolayer cultures. To explore whether cell surface glycoconjugates on these two cell lines mediate binding of H. pylori, various carbohydrates, glycoproteins, and glycolipids were tested to inhibit H.pylori cell adhesion. The adhesion was...

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Bibliographic Details
Published in:Current microbiology 1997-05, Vol.34 (5), p.267-272
Main Authors: WADSTRÖM, T, HIRMO, S, NOVAK, H, GUZMAN, A, RINGNER-PANTZAR, M, UTT, M, ALELJUNG, P
Format: Article
Language:English
Subjects:
Adhesion
Bacterial Adhesion - drug effects
Bacteriology
Biological and medical sciences
Biotechnology
Carbohydrate Metabolism
Carbohydrates
Fundamental and applied biological sciences. Psychology
Glycoconjugates - pharmacology
Glycoproteins
HeLa Cells
Helicobacter pylori - drug effects
Helicobacter pylori - metabolism
Heparin - metabolism
Humans
Infections
Microbiology
Neuraminidase - pharmacology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Periodic Acid - pharmacology
Stomach Neoplasms - microbiology
Sulfoglycosphingolipids - pharmacology
Tumor Cells, Cultured
Ulcers
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Summary:Helicobacter pylori adhere to Kato III and Hela S3 cells in monolayer cultures. To explore whether cell surface glycoconjugates on these two cell lines mediate binding of H. pylori, various carbohydrates, glycoproteins, and glycolipids were tested to inhibit H.pylori cell adhesion. The adhesion was measured (i) with a urease-based assay and (ii) by cells stained with fluorescein. Sodium periodate and sialidase treatment (but not alpha- or beta-galactosidase, heparitinase,lysozyme, or trypsin) inhibited H. pylori binding to both cell lines. Sulfatides and sulfated glycoconjugates (50 microg/ml) but not heparin or a number of simple carbohydrates inhibited binding (1 mg/ml). The two H.pylori strains studied (CCUG 17874 and strain 25) showed high binding of soluble 125I-labeled heparin and other sulfated carbohydrate compounds.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900180