Nucleosome Mobility and the Maintenance of Nucleosome Positioning

To study nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (ATP)-dependent chromatin assembly system to establish the positioning of five nucleosomes, with one nucleosome located between two R3 lac operators. When R3 protein was dissociated from DNA wi...

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Published in:Science (American Association for the Advancement of Science) 1997-05, Vol.276 (5313), p.809-812
Main Authors: Pazin, Michael J., Bhargava, Purnima, Geiduschek, E. Peter, Kadonaga, James T.
Format: Article
Language:English
Subjects:
Adenosine triphosphatases
Adenosine Triphosphate - metabolism
Apyrase - metabolism
Binding Sites
Biological and medical sciences
Chromatin
Chromatin - metabolism
Chromatin. Chromosome
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA binding proteins
DNA-Binding Proteins - metabolism
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genes
Genetic regulation
Genetic transcription
Genetics
Isopropyl Thiogalactoside - pharmacology
Mobility
Molecular and cellular biology
Molecular biology
Molecular genetics
Nucleosomes
Nucleosomes - physiology
Nucleotide sequences
Operator Regions, Genetic
Plasmids
Proteins
Regulation
Repressor proteins
Repressor Proteins - metabolism
Transcription (Genetics)
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description To study nucleosome mobility and positioning, the R3 lac repressor was used with an adenosine triphosphate (ATP)-dependent chromatin assembly system to establish the positioning of five nucleosomes, with one nucleosome located between two R3 lac operators. When R3 protein was dissociated from DNA with isopropyl β-D-thiogalactopyranoside, the R3-induced nucleosome positions remained unchanged for at least 60 minutes in the absence of ATP but rearranged within 15 minutes in the presence of ATP. These results suggest that nucleosomes are dynamic and mobile rather than static and that a DNA binding factor is continuously required for the maintenance of nucleosome positioning.
doi_str_mv 10.1126/science.276.5313.809
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subjects Adenosine triphosphatases
Adenosine Triphosphate - metabolism
Apyrase - metabolism
Binding Sites
Biological and medical sciences
Chromatin
Chromatin - metabolism
Chromatin. Chromosome
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA binding proteins
DNA-Binding Proteins - metabolism
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genes
Genetic regulation
Genetic transcription
Genetics
Isopropyl Thiogalactoside - pharmacology
Mobility
Molecular and cellular biology
Molecular biology
Molecular genetics
Nucleosomes
Nucleosomes - physiology
Nucleotide sequences
Operator Regions, Genetic
Plasmids
Proteins
Regulation
Repressor proteins
Repressor Proteins - metabolism
Transcription (Genetics)
title Nucleosome Mobility and the Maintenance of Nucleosome Positioning
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