Determination of naphthalene-2,3-dicarboxaldehyde-labeled amino acids by open tubular liquid chromatography with electrochemical detection

Naphthalene-2,3-dicarboxaldehyde (NDA) has been investigated as a new derivatizing reagent for the electrochemical detection of tagged amino acids. Gradient elution allowed for the separation of 18 NDA-derivatized amino acids on an open tubular liquid chromatography column in less than 50 min. Gradi...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1989-03, Vol.61 (5), p.432-435
Main Authors: Oates, Mary D, Jorgenson, James W
Format: Article
Language:English
Subjects:
amino acids
Amino Acids - analysis
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid
Electrochemistry
Exact sciences and technology
Hydrolysis
liquid chromatography
naphthalene-2,3-dicarboxaldehyde
Naphthalenes
Other chromatographic methods
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Description
Summary:Naphthalene-2,3-dicarboxaldehyde (NDA) has been investigated as a new derivatizing reagent for the electrochemical detection of tagged amino acids. Gradient elution allowed for the separation of 18 NDA-derivatized amino acids on an open tubular liquid chromatography column in less than 50 min. Gradient elution and electrochemical detection were found to be compatible. A detection limit of 36 amol was obtained for the asparagine-NDA derivative. The usefulness of this technique for quantitation was demonstrated by the analysis of the NDA-tagged hydrolysis products from bovine chymotrypsinogen.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00180a011