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Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection
Part of the Cε3–Cε4 region of the ovine immunoglobulin E (IgE) gene (nucleotides 1111–1575) was amplified by PCR. The recombinant protein (recIgE1-2) was expressed in E. coli and both monoclonal and polyclonal antibodies were produced. These antibodies recognized recIgE1-2 and native IgE on Western...
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| Published in: | Parasitology 1997-04, Vol.114 (4), p.395-406 |
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| Main Authors: | , , , , , |
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| Language: | English |
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| container_end_page | 406 |
| container_issue | 4 |
| container_start_page | 395 |
| container_title | Parasitology |
| container_volume | 114 |
| creator | KOOYMAN, F. N. J. VAN KOOTEN, P. J. S. HUNTLEY, J. F. MacKELLAR, A. CORNELISSEN, A. W. C. A. SCHALLIG, H. D. F. H. |
| description | Part of the Cε3–Cε4 region of the ovine immunoglobulin
E
(IgE) gene (nucleotides 1111–1575) was amplified by PCR.
The recombinant protein (recIgE1-2) was expressed in E. coli and
both
monoclonal and polyclonal antibodies were
produced. These antibodies recognized recIgE1-2 and native IgE on Western
blots
and in ELISA. The polyclonal serum
showed cross-reactivity with other sheep immunoglobulin classes. The monoclonal
antibody was specific for ovine IgE
and goat IgE. Infection of sheep with the abomasal nematode Haemonchus
contortus resulted in elevated IgE levels in
serum 2–4 weeks after infection, as measured by sandwich ELISA using
the
rabbit polyclonal as capture antibody and
the monoclonal antibody against ovine IgE as second antibody. A negative
correlation between worm counts and total
serum IgE levels at the end of the experiment was found in repeatedly infected
sheep. Significant increased levels of
excretory–secretory antigens specific IgE levels were found after
H.
contortus infection. In contrast, no significant changes
in 3rd-stage larvae (L3) antigen-specific IgE titre in sera could be detected
after infection. |
| doi_str_mv | 10.1017/S0031182096008633 |
| format | article |
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E
(IgE) gene (nucleotides 1111–1575) was amplified by PCR.
The recombinant protein (recIgE1-2) was expressed in E. coli and
both
monoclonal and polyclonal antibodies were
produced. These antibodies recognized recIgE1-2 and native IgE on Western
blots
and in ELISA. The polyclonal serum
showed cross-reactivity with other sheep immunoglobulin classes. The monoclonal
antibody was specific for ovine IgE
and goat IgE. Infection of sheep with the abomasal nematode Haemonchus
contortus resulted in elevated IgE levels in
serum 2–4 weeks after infection, as measured by sandwich ELISA using
the
rabbit polyclonal as capture antibody and
the monoclonal antibody against ovine IgE as second antibody. A negative
correlation between worm counts and total
serum IgE levels at the end of the experiment was found in repeatedly infected
sheep. Significant increased levels of
excretory–secretory antigens specific IgE levels were found after
H.
contortus infection. In contrast, no significant changes
in 3rd-stage larvae (L3) antigen-specific IgE titre in sera could be detected
after infection.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182096008633</identifier><identifier>PMID: 9107026</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge: Cambridge University Press</publisher><subject>Animals ; Antibodies, Helminth - blood ; Antibodies, Monoclonal - immunology ; Antibody Specificity ; Biological and medical sciences ; Cross Reactions ; Diseases caused by nematodes ; Duodenum - cytology ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; Haemonchiasis - immunology ; Haemonchiasis - veterinary ; Haemonchus contortus ; Helminthic diseases ; IgE ; Immunoglobulin E - blood ; Immunoglobulin E - genetics ; Immunoglobulin E - immunology ; Infectious diseases ; Medical sciences ; Miscellaneous ; nematodes ; ovine ; Parasitic diseases ; Peptide Fragments - genetics ; Peptide Fragments - immunology ; Recombinant Proteins - immunology ; Sheep ; Sheep Diseases - immunology ; Thymus Gland - cytology</subject><ispartof>Parasitology, 1997-04, Vol.114 (4), p.395-406</ispartof><rights>1997 Cambridge University Press</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-47b3b0082cf59237484ed41c9a385ff13854dbeb83cdbc09f0e8a9218f0eeaa23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182096008633/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,72960</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2665616$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9107026$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KOOYMAN, F. N. J.</creatorcontrib><creatorcontrib>VAN KOOTEN, P. J. S.</creatorcontrib><creatorcontrib>HUNTLEY, J. F.</creatorcontrib><creatorcontrib>MacKELLAR, A.</creatorcontrib><creatorcontrib>CORNELISSEN, A. W. C. A.</creatorcontrib><creatorcontrib>SCHALLIG, H. D. F. H.</creatorcontrib><title>Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Part of the Cε3–Cε4 region of the ovine immunoglobulin
E
(IgE) gene (nucleotides 1111–1575) was amplified by PCR.
The recombinant protein (recIgE1-2) was expressed in E. coli and
both
monoclonal and polyclonal antibodies were
produced. These antibodies recognized recIgE1-2 and native IgE on Western
blots
and in ELISA. The polyclonal serum
showed cross-reactivity with other sheep immunoglobulin classes. The monoclonal
antibody was specific for ovine IgE
and goat IgE. Infection of sheep with the abomasal nematode Haemonchus
contortus resulted in elevated IgE levels in
serum 2–4 weeks after infection, as measured by sandwich ELISA using
the
rabbit polyclonal as capture antibody and
the monoclonal antibody against ovine IgE as second antibody. A negative
correlation between worm counts and total
serum IgE levels at the end of the experiment was found in repeatedly infected
sheep. Significant increased levels of
excretory–secretory antigens specific IgE levels were found after
H.
contortus infection. In contrast, no significant changes
in 3rd-stage larvae (L3) antigen-specific IgE titre in sera could be detected
after infection.</description><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Cross Reactions</subject><subject>Diseases caused by nematodes</subject><subject>Duodenum - cytology</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Haemonchiasis - immunology</subject><subject>Haemonchiasis - veterinary</subject><subject>Haemonchus contortus</subject><subject>Helminthic diseases</subject><subject>IgE</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin E - genetics</subject><subject>Immunoglobulin E - immunology</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>nematodes</subject><subject>ovine</subject><subject>Parasitic diseases</subject><subject>Peptide Fragments - genetics</subject><subject>Peptide Fragments - immunology</subject><subject>Recombinant Proteins - immunology</subject><subject>Sheep</subject><subject>Sheep Diseases - immunology</subject><subject>Thymus Gland - cytology</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFksFu1DAURS0EKkPhA1ggeYHYBew4cZwlqqYzlYqgFNaW4zwPLokd7BjRf-FjcTrRbJBgYz_pnnd1rWuEXlLylhLavLslhFEqStJyQgRn7BHa0Iq3haCcPkabRS4W_Sl6FuMdIYQzXp6hs5aShpR8g35_Cr5PerbeYW-wwqN3Xg_eqQErN9vO9_c4TqCtsRobH7D_aR1gO47J-cPguzRYh7cZ7rGdI1bTNFitHgxnv9jZOW9FCGnEV4ctDhAn7yLERd4ryIT-liLW3mVwzpN1Bh4SPUdPjBoivFjvc_T1cvvlYl9cf9xdXby_LnTFxFxUTce6_P5Sm7otWVOJCvqK6lYxURtD81n1HXSC6b7TpDUEhGpLKvIASpXsHL05-k7B_0gQZznaqGEYlAOfomxEyxmt2H9ByktWN0JkkB5BHXyMAYycgh1VuJeUyKU6-Vd1eefVap66EfrTxtpV1l-vuopaDSYop208YSXnNacLVhwxG2f4dZJV-C55w5pa8t2NvPnc7m73Yi8_ZJ6tUdXYBdsfQN75FPIHiP8I-wfhUsKH</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>KOOYMAN, F. N. J.</creator><creator>VAN KOOTEN, P. J. S.</creator><creator>HUNTLEY, J. F.</creator><creator>MacKELLAR, A.</creator><creator>CORNELISSEN, A. W. C. A.</creator><creator>SCHALLIG, H. D. F. H.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19970401</creationdate><title>Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection</title><author>KOOYMAN, F. N. J. ; VAN KOOTEN, P. J. S. ; HUNTLEY, J. F. ; MacKELLAR, A. ; CORNELISSEN, A. W. C. A. ; SCHALLIG, H. D. F. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-47b3b0082cf59237484ed41c9a385ff13854dbeb83cdbc09f0e8a9218f0eeaa23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Cross Reactions</topic><topic>Diseases caused by nematodes</topic><topic>Duodenum - cytology</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Haemonchiasis - immunology</topic><topic>Haemonchiasis - veterinary</topic><topic>Haemonchus contortus</topic><topic>Helminthic diseases</topic><topic>IgE</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin E - genetics</topic><topic>Immunoglobulin E - immunology</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Miscellaneous</topic><topic>nematodes</topic><topic>ovine</topic><topic>Parasitic diseases</topic><topic>Peptide Fragments - genetics</topic><topic>Peptide Fragments - immunology</topic><topic>Recombinant Proteins - immunology</topic><topic>Sheep</topic><topic>Sheep Diseases - immunology</topic><topic>Thymus Gland - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOOYMAN, F. N. J.</creatorcontrib><creatorcontrib>VAN KOOTEN, P. J. S.</creatorcontrib><creatorcontrib>HUNTLEY, J. F.</creatorcontrib><creatorcontrib>MacKELLAR, A.</creatorcontrib><creatorcontrib>CORNELISSEN, A. W. C. A.</creatorcontrib><creatorcontrib>SCHALLIG, H. D. F. H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOOYMAN, F. N. J.</au><au>VAN KOOTEN, P. J. S.</au><au>HUNTLEY, J. F.</au><au>MacKELLAR, A.</au><au>CORNELISSEN, A. W. C. A.</au><au>SCHALLIG, H. D. F. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>114</volume><issue>4</issue><spage>395</spage><epage>406</epage><pages>395-406</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Part of the Cε3–Cε4 region of the ovine immunoglobulin
E
(IgE) gene (nucleotides 1111–1575) was amplified by PCR.
The recombinant protein (recIgE1-2) was expressed in E. coli and
both
monoclonal and polyclonal antibodies were
produced. These antibodies recognized recIgE1-2 and native IgE on Western
blots
and in ELISA. The polyclonal serum
showed cross-reactivity with other sheep immunoglobulin classes. The monoclonal
antibody was specific for ovine IgE
and goat IgE. Infection of sheep with the abomasal nematode Haemonchus
contortus resulted in elevated IgE levels in
serum 2–4 weeks after infection, as measured by sandwich ELISA using
the
rabbit polyclonal as capture antibody and
the monoclonal antibody against ovine IgE as second antibody. A negative
correlation between worm counts and total
serum IgE levels at the end of the experiment was found in repeatedly infected
sheep. Significant increased levels of
excretory–secretory antigens specific IgE levels were found after
H.
contortus infection. In contrast, no significant changes
in 3rd-stage larvae (L3) antigen-specific IgE titre in sera could be detected
after infection.</abstract><cop>Cambridge</cop><pub>Cambridge University Press</pub><pmid>9107026</pmid><doi>10.1017/S0031182096008633</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-1820 |
| ispartof | Parasitology, 1997-04, Vol.114 (4), p.395-406 |
| issn | 0031-1820 1469-8161 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78963143 |
| source | Cambridge Journals Online |
| subjects | Animals Antibodies, Helminth - blood Antibodies, Monoclonal - immunology Antibody Specificity Biological and medical sciences Cross Reactions Diseases caused by nematodes Duodenum - cytology ELISA Enzyme-Linked Immunosorbent Assay - methods Haemonchiasis - immunology Haemonchiasis - veterinary Haemonchus contortus Helminthic diseases IgE Immunoglobulin E - blood Immunoglobulin E - genetics Immunoglobulin E - immunology Infectious diseases Medical sciences Miscellaneous nematodes ovine Parasitic diseases Peptide Fragments - genetics Peptide Fragments - immunology Recombinant Proteins - immunology Sheep Sheep Diseases - immunology Thymus Gland - cytology |
| title | Production of a monoclonal antibody specific for ovine immunoglobulin E and its application to monitor serum IgE responses to Haemonchus contortus infection |
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