Isolation and establishment in in vitro culture of a Theileria annulata : infected cell line from Spain

The isolation of Theileria annulata-infected lymphocytes using blood from an animal suffering from Mediterranean theileriosis as a source of parasites is described. The present work reports the first isolation and establishment in in vitro culture of a T. annulata-infected cell line from southwester...

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Bibliographic Details
Published in:Parasitology research (1987) 1997, Vol.83 (4), p.394-396
Main Authors: VISERAS, J, GARCIA-FERNANDEZ, P, ADROHER, F. J
Format: Article
Language:English
Subjects:
Animal protozoal diseases
Animals
Biological and medical sciences
Cattle
Cell Line - parasitology
Female
Glucose-6-Phosphate Isomerase - analysis
Infectious diseases
Isoenzymes - analysis
Lymphocytes - cytology
Lymphocytes - parasitology
Medical sciences
Parasitic diseases
Polymorphism, Genetic
Protozoal diseases
Spain
Species Specificity
Theileriasis - enzymology
Theileriasis - parasitology
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Description
Summary:The isolation of Theileria annulata-infected lymphocytes using blood from an animal suffering from Mediterranean theileriosis as a source of parasites is described. The present work reports the first isolation and establishment in in vitro culture of a T. annulata-infected cell line from southwestern Europe, where Mediterranean theileriosis causes important economic losses, especially in southern Spain. The parasite was identified by staining of cells from culture with Giemsa, by immunofluorescent antibody techniques (IFAT), and by isoenzyme characterization. The possibility of using this T. annulata-infected lymphoblastoid cell line to obtain an antigen for diagnosis of Mediterranean theileriosis by IFAT and to develop a tissue-culture vaccine against this disease in our geographic area shows the significance of this isolation and culture.
ISSN:0932-0113
1432-1955
DOI:10.1007/s004360050270