The `sniffer-patch' technique for detection of neurotransmitter release

A wide variety of techniques have been employed for the detection and measurement of neurotransmitter release from biological preparations. Whilst many of these methods offer impressive levels of sensitivity, few are able to combine sensitivity with the necessary temporal and spatial resolution requ...

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Bibliographic Details
Published in:Trends in neurosciences (Regular ed.) 1997-05, Vol.20 (5), p.192-197
Main Author: Allen, T.G.J.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques
Calibration
Fundamental and applied biological sciences. Psychology
General aspects. Models. Methods
Humans
Medical research
Neurology
Neurosciences - methods
Neurotransmitter Agents - metabolism
Sensors
Time Factors
Vertebrates: nervous system and sense organs
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Summary:A wide variety of techniques have been employed for the detection and measurement of neurotransmitter release from biological preparations. Whilst many of these methods offer impressive levels of sensitivity, few are able to combine sensitivity with the necessary temporal and spatial resolution required to study quantal release from single cells. One detection method that is seeing a revival of interest and has the potential to fill this niche is the so-called `sniffer-patch' technique. In this article, specific examples of the practical aspects of using this technique are discussed along with the procedures involved in calibrating these biosensors to extend their applications to provide quantitative, in addition to simple qualitative, measurements of quantal transmitter release.
ISSN:0166-2236
1878-108X
DOI:10.1016/S0166-2236(96)01039-9