Effects of Exogenous and Endogenous Nitric Oxide on the Contractile Function of Cultured Chick Embryo Ventricular Myocytes

• Published in: Journal of molecular and cellular cardiology 1997-02, Vol.29 (2), p.677-687
• Main Authors: Ungureanu-Longrois, Dan, Bézie, Yvonnick, Perret, Claudine, Laurent, Stéphane
• Format: Article
• Language: English
• Subjects: Adrenergic beta-Agonists - pharmacology; Animals; Arginine - metabolism; Arginine - pharmacology; Calcium - metabolism; Calcium - pharmacology; Chick Embryo; Chick embryo ventricular myocytes; Cyclic AMP - chemistry; Cyclic AMP - metabolism; Ferrocyanides - pharmacology; Heart Ventricles - cytology; Heart Ventricles - embryology; Isoproterenol - pharmacology; l-arginine; Myocardial Contraction - drug effects; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide - pharmacology; Nitric Oxide Synthase - metabolism; Nitroprusside - pharmacology; Nucleotides, Cyclic - chemistry; Nucleotides, Cyclic - metabolism; Sodium nitroprusside; Substrate Specificity; Vasodilator Agents - pharmacology
• ISSN: 0022-2828; 1095-8584
• DOI: 10.1006/jmcc.1996.0310

Nitric oxide (NO) has been shown to be a ubiquitous intercellular autacoid in the heart and, in cultured rat ventricular myocytes, to decrease the contractile responsiveness to isoproterenol (ISO). The aim of the present study was to investigate whether exogenous (sodium nitroprusside, SNP) or endogenous nitric oxide generated froml-arginine modulated the response to ISO in cultured chick embryo ventricular myocytes. SNP 1μmorl-arginine 1 mmhad no effect on baseline contractile function. Superfusion with ISO 100 nmsignificantly increased myocyte amplitude of shortening to 1.31±0.06 (ratio to baseline amplitude). Initial superfusion with SNP 1μmorl-arginine 1 mmattenuated the response to ISO to 0.89±0.05 and 1.09±0.07 respectively (P