Detection of an antigenic protein of Leptospira interrogans which shares epitopes with the equine cornea and lens

A protein epitope which is involved in an antigenic relationship between equine ocular tissues and Leptospira interrogans was detected in homogenates of the bacterium. The antigenic determinant was harboured on a peptide structure which was shown to be sensitive to the action of denaturing and reduc...

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Bibliographic Details
Published in:The veterinary journal (1997) 1997-01, Vol.153 (1), p.75-79
Main Authors: Parma, A E, Sanz, M E, Lucchesi, P M, Mazzonelli, J, Petruccelli, M A
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - analysis
Antigens, Bacterial - immunology
Bacterial Proteins - immunology
Blotting, Western - veterinary
Cornea - immunology
Cross Reactions
Electrophoresis, Polyacrylamide Gel - veterinary
Epitopes - immunology
Horses - immunology
Immunohistochemistry
Lens, Crystalline - immunology
Leptospira interrogans - immunology
Leptospira interrogans - ultrastructure
Microscopy, Electron - veterinary
Rabbits
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Description
Summary:A protein epitope which is involved in an antigenic relationship between equine ocular tissues and Leptospira interrogans was detected in homogenates of the bacterium. The antigenic determinant was harboured on a peptide structure which was shown to be sensitive to the action of denaturing and reducing agents by means of Western blotting. The outer surface of the leptospires appeared to be free of this epitope as was proved by dot-blot and electron microscopic studies.
ISSN:1090-0233
DOI:10.1016/S1090-0233(97)80011-1