Ontogeny of Glutamic Acid Decarboxylase Gene Expression in the Mouse Pancreas

• Published in: Biochemical and biophysical research communications 1997-04, Vol.233 (1), p.227-230
• Main Authors: Pleau, Jean-Marie, Throsby, Mark, Esling, Anne, Dardenne, Mireille
• Format: Article
• Language: English
• Subjects: Animals; Female; Gene Expression Regulation, Developmental; Glutamate Decarboxylase - genetics; Isoenzymes - genetics; Male; Mice; Mice, Inbred C57BL; Mice, Inbred NOD; Pancreas - embryology; Pancreas - enzymology; Pancreas - growth & development
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1997.6432

Using two RT–PCR quantitative assays, we measured the pancreatic expression of the two isoforms of glutamic acid decarboxylase in the foetus, newborn and 14-, 21- and 35-day-old male and female NOD and C57BL/6 mice. In the C57BL/6 mouse, GAD 67 pancreatic expression is stable; in NOD mice, GAD 67 expression is similar to that found in control mice, except at 5 weeks of age, when pancreatic GAD 67 expression is about 2.5 times higher than in C57BL/6 mice. The pancreatic expression of GAD 65 is under the detection limit of the assay until 5 weeks of age. The overexpression of GAD 67 characterized in pancreas from 5-week-old NOD mice could be the result of β cell hyperactivity, previously reported in this mouse strain.