Further characterization of hla homozygous typing cell lines at the LMP2 polymorphic codon 60 by an ARMS typing method

LMP2 is a subunit of the 20S proteasome within the cellular cytosolic compartment that is thought to cleave proteins into approximately 9 amino acid long oligopeptides. It is hypothesized that changes in the low molecular mass protease (LMP) gene sequence may alter the activity or specificity in whi...

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Bibliographic Details
Published in:Human immunology 1997-04, Vol.53 (2), p.183-187
Main Authors: Hopkins, Leann M., Bull, Peggy J., Gerlach, John A., Bull, Robert W.
Format: Article
Language:English
Subjects:
Cell Line
Codon - genetics
Cysteine Endopeptidases
DNA Primers
Genotype
Histocompatibility Testing - methods
HLA Antigens - genetics
Homozygote
Humans
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Proteins - genetics
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Summary:LMP2 is a subunit of the 20S proteasome within the cellular cytosolic compartment that is thought to cleave proteins into approximately 9 amino acid long oligopeptides. It is hypothesized that changes in the low molecular mass protease (LMP) gene sequence may alter the activity or specificity in which the LMP genes cleave peptides. Currently, the typing method for LMP2 involves polymerase chain reaction (PCR), restriction enzyme digestion, and gel electrophoresis. To help reduce the cost and cumbersomeness of this method, a new typing method was adapted for the LMP2 gene. To establish this new amplification refractory mutation system (ARMS) typing method, primers have been defined, amplification conditions optimized, and control cell lines sequenced to validate testing parameters. Results are listed for selected 10th and 11th International Histocompatibility Workshop homozygous cell lines.
ISSN:0198-8859
1879-1166
DOI:10.1016/S0198-8859(97)00036-0