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Polymerase Chain Reaction Analysis of Distal Vaginal Specimens: A Less Invasive Strategy for Detection of Trichomonas vaginalis

We compared polymerase chain reaction (PCR) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. One or all three techniques revealed that 61 (20.3%) of 300 women tested were positive for Trichomonas vaginalis. PC...

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Published in:	Clinical infectious diseases 1997-05, Vol.24 (5), p.985-987
Main Authors:	Heine, R. Phillips, Wiesenfeld, Harold C., Sweet, Richard L., Witkin, Steven S.
Format:	Article
Language:	English
Subjects:	Animals Biological and medical sciences Cell Culture Techniques Evaluation Studies as Topic Female General aspects Gynecological examination Gynecology Human infectious diseases. Experimental studies and models Humans Infections Infectious diseases Medical sciences Microscopy Microscopy - methods Obstetrics Polymerase Chain Reaction Sensitivity and Specificity Sexually transmitted diseases Specimens Surgical instruments Trichomonas vaginalis Trichomonas vaginalis - isolation & purification Trichomonas Vaginitis - diagnosis Vagina Vagina - cytology Vagina - parasitology Vaginal Smears
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container_end_page	987
container_issue	5
container_start_page	985
container_title	Clinical infectious diseases
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creator	Heine, R. Phillips Wiesenfeld, Harold C. Sweet, Richard L. Witkin, Steven S.
description	We compared polymerase chain reaction (PCR) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. One or all three techniques revealed that 61 (20.3%) of 300 women tested were positive for Trichomonas vaginalis. PCR analysis of distal vaginal specimens detected 56 (91.8%) of 61 infections, while wet mount microscopy and culture detected 49 (80.3%) of 61 infections. Results of this study may impact the approach to testing for T. vaginalis by eliminating the requirement of a vaginal speculum examination. The distal vagina is an appropriate testing site for T. vaginalis by PCR analysis.
doi_str_mv	10.1093/clinids/24.5.985
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source	JSTOR Archival Journals and Primary Sources Collection; Oxford Journals Online
subjects	Animals Biological and medical sciences Cell Culture Techniques Evaluation Studies as Topic Female General aspects Gynecological examination Gynecology Human infectious diseases. Experimental studies and models Humans Infections Infectious diseases Medical sciences Microscopy Microscopy - methods Obstetrics Polymerase Chain Reaction Sensitivity and Specificity Sexually transmitted diseases Specimens Surgical instruments Trichomonas vaginalis Trichomonas vaginalis - isolation & purification Trichomonas Vaginitis - diagnosis Vagina Vagina - cytology Vagina - parasitology Vaginal Smears
title	Polymerase Chain Reaction Analysis of Distal Vaginal Specimens: A Less Invasive Strategy for Detection of Trichomonas vaginalis
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