28s rDNA group-I introns: a powerful tool for identifying strains of Beauveria brongniartii

The nuclear ribosomal DNA of the entomopathogenic fungus Beauveria brongniartii is polymorphic in terms of both restriction site and length. Insertions of 350–450 bp long, identified as group‐I introns, were detected in the 28s rDNA. A panel of 47 strains of B. brongniartii, two B. bassiana and one...

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Bibliographic Details
Published in:Molecular ecology 1997-04, Vol.6 (4), p.373-381
Main Authors: Neuvéglise, C., Brygoo, Y., Riba, G.
Format: Article
Language:English
Subjects:
28s rDNA
Animals
Base Sequence
Beauveria
Beauveria bassiana
Beauveria brongniartii
Coleoptera - microbiology
detection
DNA Primers - genetics
DNA Transposable Elements
DNA, Fungal - genetics
DNA, Ribosomal - genetics
entomopathogenic fungus
Genetic Variation
group-I introns
Introns
Metarhizium anisopliae
Mitosporic Fungi - genetics
Mitosporic Fungi - isolation & purification
Mitosporic Fungi - pathogenicity
Pest Control, Biological
RNA, Fungal - genetics
RNA, Ribosomal, 28S - genetics
Saccharum officinarum
Scarabaeidae
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Summary:The nuclear ribosomal DNA of the entomopathogenic fungus Beauveria brongniartii is polymorphic in terms of both restriction site and length. Insertions of 350–450 bp long, identified as group‐I introns, were detected in the 28s rDNA. A panel of 47 strains of B. brongniartii, two B. bassiana and one Metarhizium anisopliae of various geographical and biological origins were found to contain 14 variant forms of intron differing in size and restriction pattern, at four different positions. Twelve types of ribosomal large subunit were defined on the basis of variant distribution and compared with strain clustering based on internal transcribed spacers analysis. There was a correlation between the characteristic introns and isolates collected from the sugar cane pest Hoplochelus marginalis. Primers for polymerase chain reaction amplification were chosen from these variants, and used to develop a specific method for detecting strains pathogenic towards Hoplochelus.
ISSN:0962-1083
1365-294X
DOI:10.1046/j.1365-294X.1997.00202.x