Does the secretion of cytokines in the periphery reflect their role in bone metabolic diseases?

• Published in: Mechanisms of ageing and development 1997-03, Vol.94 (1), p.17-23
• Main Authors: Natale, V.M, Jacob Filho, W, Duarte, A.J.S
• Format: Article
• Language: English
• Subjects: Aged; Aging; Biological and medical sciences; Bone Diseases, Metabolic - metabolism; Bone remodeling diseases; Diseases of the osteoarticular system; Female; Humans; Interleukin-1; Interleukin-1 - metabolism; Interleukin-6; Interleukin-6 - metabolism; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - metabolism; Male; Medical sciences; Osteitis Deformans - metabolism; Osteoporosis; Osteoporosis - metabolism; Osteoporosis. Osteomalacia. Paget disease; Paget's bone disease
• ISSN: 0047-6374; 1872-6216
• DOI: 10.1016/S0047-6374(96)01844-1

Much interest has been focused on the role of the immune system in bone remodeling. Here, we compare the production of Interleukin-1 β (IL-1 β) and Interleukin-6 (IL-6) by peripheral blood mononuclear cells (PBMC) in elderly Paget's patients, elderly osteoporotic patients and in normal elderly subjects. We studied Paget's patients (71.00±3.74 years), 7 osteoporotic patients (71.86±3.23 years), age and sex matched, and 5 elderly healthy control subjects (74.20±4.10 years) An ELISA test was used to quantify IL-1 β and IL-6 in the supernatant culture of PBMC stimulated by phytohemagglutinin (PHA). IL-1 β and IL-6 production from Paget's patients (IL-1 β, 651.43±95.92 pg/ml; IL-6, 1402.85±148.11 pg/ml) was not statistically different from the production observed in the osteoporotic patients (IL-1 β, 552.57±79.04 pg/ml; IL-6, 1458.85±118.35 pg/ml) and in the healthy elderly group (IL-1 β, 717.60±131.34 pg/ml; IL-6, 1502.40±211.90 pg/ml). Although IL-1 and IL-6 can be involved in the bone remodeling process, we did not find any difference when we compared their production by PBMC in elderly normal, elderly osteoporotic and elderly Paget's patients.