Rescue of a Drosophila NF1 mutant phenotype by protein kinase A

The neurofibromatosis type 1 (NF1) tumor suppressor protein is thought to restrict cell proliferation by functioning as a Ras-specific guanosine triphosphatase-activating protein. However, Drosophila homozygous for null mutations of an NF1 homolog showed no obvious signs of perturbed Ras1-mediated s...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1997-05, Vol.276 (5313), p.791-794
Main Authors: The, I. (Massachusetts General Hospital Cancer Center, Charlestown, MA.), Hannigan, G.E, Cowley, G.S, Reginald, S, Zhong, Y, Gusella, J.F, Hariharan, I.K, Bernards, A
Format: Article
Language:English
Subjects:
Alleles
Amino Acid Sequence
Animals
Biological and medical sciences
Cell Count
Classical genetics, quantitative genetics, hybrids
Complementary DNA
Cyclic AMP - metabolism
Cyclic AMP-Dependent Protein Kinases - genetics
Cyclic AMP-Dependent Protein Kinases - metabolism
Developmental Stages
DROSOPHILA
Drosophila - cytology
Drosophila - genetics
Drosophila - growth & development
Drosophila - metabolism
Drosophila Proteins
Epidermal cells
Exons
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genes, Insect
Genetic aspects
Genetics of eukaryotes. Biological and molecular evolution
Genomics
Genotypes
GTP Phosphohydrolases - metabolism
Individualized Instruction
Insect Proteins - chemistry
Insect Proteins - genetics
Insect Proteins - metabolism
Insects
Invertebrata
Molecular Sequence Data
MUTACION
MUTATION
Nerve Tissue Proteins
Neurofibromin 1
Phenotype
Phenotypes
Protein kinases
PROTEINAS
PROTEINE
Proteins
Proteins - chemistry
Proteins - genetics
Pupae
ras GTPase-Activating Proteins
ras Proteins - metabolism
Recombinant Fusion Proteins - pharmacology
Signal Transduction
Transgenes
Tumors
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Description
Summary:The neurofibromatosis type 1 (NF1) tumor suppressor protein is thought to restrict cell proliferation by functioning as a Ras-specific guanosine triphosphatase-activating protein. However, Drosophila homozygous for null mutations of an NF1 homolog showed no obvious signs of perturbed Ras1-mediated signaling. Loss of NF1 resulted in a reduction in size of larvae, pupae, and adults. This size defect was not modified by manipulating Ras1 signaling but was restored by expression of activated adenosine 3',5'-monophosphate-dependent protein kinase (PKA). Thus, NF1 and PKA appear to interact in a pathway that controls the overall growth of Drosophila
ISSN:0036-8075
1095-9203
DOI:10.1126/science.276.5313.791