Genome sizes of the entomopathogenic nematodes Steinernema carpocapsae and Heterorhabditis bacteriophora (Nematoda: Rhabditida)

There is currently no information on the genome size and complexity in the entomoparasitic nematodes Steinernema and Heterorhabditis. DNA reassociation kinetics were used to determine the genome size and complexity in 2 species: Steinernema carpocapsae and Heterorhabditis bacteriophora. C0t curves d...

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Bibliographic Details
Published in:Parasitology 1997-05, Vol.114 (5), p.497-501
Main Authors: GRENIER, E., CATZEFLIS, F. M., ABAD, P.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
C0 t curves
Cytogenetics
DNA complexity
DNA, Helminth - chemistry
DNA, Helminth - genetics
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Genome
Heterorhabditis bacteriophora
Invertebrata
Kinetics
Molecular Weight
Nucleic Acid Renaturation
Repetitive Sequences, Nucleic Acid
Rhabditoidea - genetics
Steinernema carpocapsae
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Summary:There is currently no information on the genome size and complexity in the entomoparasitic nematodes Steinernema and Heterorhabditis. DNA reassociation kinetics were used to determine the genome size and complexity in 2 species: Steinernema carpocapsae and Heterorhabditis bacteriophora. C0t curves derived from renaturation kinetics of denatured DNA indicate that the genomes of these entomoparasitic rhabditids are different both in size and complexity. Genome sizes were estimated at 2·3 × 108 bp for S. carpocapsae and 3·9 × 107 bp for H. bacteriophora and repetitive DNA contents were found to represent 39% and 51% of these respective genomes.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182096008736