The Interaction between Human Cytomegalovirus Immediate-Early Gene 2 (IE2) Protein and Heterogeneous Ribonucleoprotein A1

Human cytomegalovirus (HCMV) is an ubiquitous pathogen which causes significant illness in immunocompromised individuals. The immediate-early gene 2 (IE2) protein of HCMV plays an important role in the regulation of virus replication. Previous studies have shown that the IE2 protein is able to inter...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1997-03, Vol.232 (3), p.590-594
Main Authors: Wang, Yi-Fen, Chen, Shan-Chun, Wu, Felicia Y.-H., Wu, Cheng-Wen
Format: Article
Language:English
Subjects:
Base Sequence
Binding Sites
Cytomegalovirus - genetics
Cytomegalovirus - metabolism
Cytomegalovirus - pathogenicity
DNA Primers - genetics
Heterogeneous Nuclear Ribonucleoprotein A1
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Heterogeneous-Nuclear Ribonucleoproteins
Humans
Immediate-Early Proteins - genetics
Immediate-Early Proteins - metabolism
Membrane Glycoproteins
Protein Binding
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Ribonucleoproteins - metabolism
Saccharomyces cerevisiae - genetics
Trans-Activators
Viral Envelope Proteins
Viral Proteins
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Summary:Human cytomegalovirus (HCMV) is an ubiquitous pathogen which causes significant illness in immunocompromised individuals. The immediate-early gene 2 (IE2) protein of HCMV plays an important role in the regulation of virus replication. Previous studies have shown that the IE2 protein is able to interact with several cellular proteins, but many of the IE2 interacting partners remain unidentified. By utilizing the yeast two-hybrid system, the heterogeneous ribonucleoprotein A1 (hnRNP A1) was identified as an IE2 interacting protein. The interaction was confirmed via thein vitrobinding of bacterial expressed glutathioneS-transferase (GST) IE2 fusion protein with thein vitrotranslated hnRNP A1. The mutational analysis showed that both the N-terminal half (1-290 residues) and C-terminal half (291-579 residues) of IE2 protein can interact with the hnRNP A1, indicating that more than one region of IE2 protein are involved in the binding with hnRNP A1.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.6334