The human bladder carcinoma cell line 5637 constitutively secretes functional cytokines

• Published in: Leukemia research 1997-04, Vol.21 (4), p.343-350
• Main Authors: Quentmeier, Hilmar, Zaborski, Margarete, Drexler, Hans G.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Carcinoma - metabolism; Carcinoma - pathology; cell line; Cytokines; Cytokines - biosynthesis; Enzyme-Linked Immunosorbent Assay; G-CSF; GM-CSF; Granulocyte Colony-Stimulating Factor - biosynthesis; Granulocyte-Macrophage Colony-Stimulating Factor - biosynthesis; Humans; IL-1; M-CSF; Medical sciences; Nephrology. Urinary tract diseases; SCF; Tumor Cells, Cultured; Tumors of the urinary system; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; Urinary tract. Prostate gland
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/S0145-2126(96)00132-4

We have studied cytokine secretion by the human bladder carcinoma cell line 5637 using growth factor-dependent cell line bioassays and specific ELISA. The 5637 cell line-conditioned medium (5637 CM) stimulated proliferation of human growth factor-dependent leukemia cell lines in a dose-dependent fashion. Quantitation by specific ELISA and by bioassays detected high amounts of G-CSF and GM-CSF and smaller quantities of IL-1β, M-CSF and SCF in 5637 CM; the concentration of IL-3 was below the detection level of the ELISA, if present at all. The G-CSF and GM-CSF activities secreted by 5637 cells could be inhibited specifically by neutralizing anti-G-CSF and anti-GM-CSF antibodies. In conclusion, 5637 bladder carcinoma cells constitutively produce and secrete several functionally active cytokines; 5637 CM is a valuable, reliable and inexpensive source for cytokines, for instance for the culture of growth factor-responsive or -dependent hematopoietic primary cells and cell lines.