Rodents infected with Schistosoma mansoni produce cytolytic IgG and IgM antibodies to the Lewis x antigen

Schistosoma mansoni is a blood fluke that produces glyco-conjugates containing the Lewis x antigen (Lex) Galβ1→4(Fucα1→3) GlcNAcβ1→R. However, Lex antigen is also normally expressed in many tissues of adult rodents. We now report that mice and hamsters chronically infected with S.mansoni generate hi...

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Bibliographic Details
Published in:Glycobiology (Oxford) 1997-03, Vol.7 (2), p.207-215
Main Authors: Nyame, A.Kwame, Pilcher, Joy B., Tsang, Victor C.W., Cummings, Richard D.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - immunology
Carbohydrate Sequence
Chronic Disease
Complement System Proteins - immunology
COS Cells
Cricetinae
HL-60 Cells
Humans
IgG
IgM
Immunoglobulin G - blood
Immunoglobulin Isotypes - blood
Immunoglobulin M - blood
Lewis x
Lewis X Antigen - immunology
Mice
Molecular Sequence Data
rodent
Schistosoma mansoni
Schistosomatidae
Schistosomiasis mansoni - immunology
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Summary:Schistosoma mansoni is a blood fluke that produces glyco-conjugates containing the Lewis x antigen (Lex) Galβ1→4(Fucα1→3) GlcNAcβ1→R. However, Lex antigen is also normally expressed in many tissues of adult rodents. We now report that mice and hamsters chronically infected with S.mansoni generate high titers of both IgM and IgG antibodies reactive with Lex and that no reactivity is present in sera from uninfected animals. Anti-Lex antibodies were detected by ELISA using the Lex-containing neoglycoprotein lacto-N-fucopentaoseIII-BSA. The IgG in infected animals consists of IgG1, IgG2a, and IgG2b subclasses and binds to Protein A-Sepharose. The sera of infected animals reacts only with Lex antigen and has no reactivity toward either Lex or sialyl Lex. The IgM response to Lex is detectable at week 2, whereas the IgG response is detectable at weeks 5–6 following infection of mice. The sera of infected mice and hamsters can mediate the complement-dependent cytolysis (CDC) of cells expressing surface Lex. This cytolytic activity is exclusively effected by the anti-Lex antibodies, since their removal from sera by adsorption depletes the sera of CDC activity. Thus, the abundant expression of the Lex antigens by the parasite elicits cytolytic antibodies reactive with a host antigen.
ISSN:0959-6658
1460-2423
DOI:10.1093/glycob/7.2.207