Frequent loss of heterozygosity of chromosome 3 short arm detected by PCR-based microsatellite polymorphisms in cervical squamous cell carcinoma

Karyotypic studies have shown that genetic aberrations of the short arm of chromosome 3 (3p) may be involved in the pathogenesis of cervical carcinoma. In this study we analyzed nine polymorphic microsatellite repeats on 3p using a PCR-based assay for loss of heterozygosity (LOH) in 64 invasive squa...

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Bibliographic Details
Published in:Cancer letters 1997-05, Vol.115 (2), p.161-164
Main Authors: Wong, Y.F., Chung, T.K.H., Cheung, T.H., Tam, P.O.S., Chang, A.M.Z.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - pathology
Cervical carcinoma
Chromosomes, Human, Pair 3
DNA, Neoplasm - genetics
DNA, Satellite - genetics
Female
Female genital diseases
Gene Deletion
Gynecology. Andrology. Obstetrics
Heterozygosity
Heterozygote
Humans
Medical sciences
Microsatellite
Neoplasm Invasiveness
Polymerase Chain Reaction
Polymorphism, Genetic
Repetitive Sequences, Nucleic Acid
Tumors
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - pathology
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Summary:Karyotypic studies have shown that genetic aberrations of the short arm of chromosome 3 (3p) may be involved in the pathogenesis of cervical carcinoma. In this study we analyzed nine polymorphic microsatellite repeats on 3p using a PCR-based assay for loss of heterozygosity (LOH) in 64 invasive squamous cell carcinomas of the cervix. These markers encompass chromosome region 3p13–25. LOH at one or more loci was detected in 46 (79%) out of the 58 informative cases. The incidence of LOH at locus D3S643 (3p13) was the highest among nine markers examined. The difference between the frequency of LOH at D3S643 in early stage (I–II) disease (43%) and those with advanced stage (stage III–IV) (79%) was statistically significant ( P < 0.05). The results indicate that tumor suppressor gene(s) that play a role in cervical cancer may be located on the short arm of chromosome 3, likely near or at 3p13. The LOH at 3p13 appears to be a late event in tumor progression and may serve as an indicator for a less favorable clinical outcome.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(97)04723-X