Cultured retinal pericytes stimulate in vitro angiogenesis of endothelial cells through secretion of a fibroblast growth factor-like molecule

Interaction between cultured endothelial cells (EC) and pericytes (PC) was studied in vitro to clarify the mechanism of diabetic proliferative retinopathy. Conditioned medium (CM) from retinal PC strongly increased the proliferation and moderately stimulated migration of retinal EC. Moreover, CM fro...

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Bibliographic Details
Published in:Atherosclerosis 1997-04, Vol.130 (1), p.101-107
Main Authors: Watanabe, Satoe, Morisaki, Nobuhiro, Tezuka, Mariko, Fukuda, Kuniaki, Ueda, Shirou, Koyama, Noriyuki, Yokote, Koutaro, Kanzaki, Tetsuto, Yoshida, Sho, Saito, Yasushi
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Basic fibroblast growth factor
Biological and medical sciences
Blood vessels and receptors
Cell Division
Cell Movement
Cells, Cultured
Coculture Techniques
Culture Media, Conditioned
Diabetes
Endothelial cells
Endothelium, Vascular - cytology
Endothelium, Vascular - physiopathology
Fibroblast Growth Factors - metabolism
Fibroblast Growth Factors - physiology
Fundamental and applied biological sciences. Psychology
Male
Neovascularization, Pathologic - physiopathology
Pericytes
Rabbits
Retina
Retinal Vessels - cytology
Retinal Vessels - metabolism
Transforming Growth Factor beta - physiology
Vertebrates: cardiovascular system
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Summary:Interaction between cultured endothelial cells (EC) and pericytes (PC) was studied in vitro to clarify the mechanism of diabetic proliferative retinopathy. Conditioned medium (CM) from retinal PC strongly increased the proliferation and moderately stimulated migration of retinal EC. Moreover, CM from PC caused stimulation of angiogenesis of retinal EC and umbilical cord vein EC in vitro at the same extent as basic fibroblast growth factor (bFGF). PC also stimulated angiogenesis by EC in mixed cultures. The angiogenic, proliferative and migration activities in CM from PC were inhibited by an antibody to bFGF. These data suggest that PC play an important role in angiogenesis through secretion of an FGF-like molecule.
ISSN:0021-9150
1879-1484
DOI:10.1016/S0021-9150(96)06050-9