Mapping of the target antigens of the rotavirus-specific cytotoxic T cell response

The cytotoxic T cell (CTL) response in C57/Bl6(H-2b) mice to rotavirus has been analysed using a cognate set of vaccinia virus recombinants covering the 12 primary gene products of the UKtc strain of bovine rotavirus. The gene products of RNA segments 5 (VP5/NSP-1) and 8 (VP7) both elicited a classi...

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Bibliographic Details
Published in:Journal of general virology 1997-05, Vol.78 (5), p.1065-1075
Main Authors: Heath, R.R, Stagg, S, Xu, F, McCrae, M.A
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - genetics
Antigens, Viral - immunology
bovine rotavirus
Capsid - genetics
Capsid - immunology
Capsid Proteins
Cattle
Cell Line
cytotoxic T-lymphocytes
Epitope Mapping
epitopes
Epitopes, T-Lymphocyte - immunology
Gene Expression
Genetic Vectors
Mice
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Recombination, Genetic
Rotavirus
Rotavirus - immunology
T-Lymphocytes, Cytotoxic - immunology
Vaccinia virus - genetics
viral antigens
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Summary:The cytotoxic T cell (CTL) response in C57/Bl6(H-2b) mice to rotavirus has been analysed using a cognate set of vaccinia virus recombinants covering the 12 primary gene products of the UKtc strain of bovine rotavirus. The gene products of RNA segments 5 (VP5/NSP-1) and 8 (VP7) both elicited a classic CD8+ Class I MHC restricted CTL response. Using L cells transfected with specific Class I MHC loci as targets the VP5/NSP-1 response was found to be restricted at Db and the VP7 response at Kb. Vaccinia virus recombinants expressing VP7 genes from seven G serotypes were used to show that the CTL response to this antigen is completely cross-reactive. By contrast, using the same strategy the CTL response to VP5/NSP-1 was found to be virus strain specific. A vaccinia virus recombinant carrying RNA segment 5 from the deletion mutant P9Ddelta5 was used to localize at least one CTL epitope in VP5/NSP-1 to the first 150 amino acids of the protein. The expression of a number of fragments of VP7 in vaccinia virus recombinants was used to show that the CTL epitope (amino acids 31-40) previously identified through the use of synthetic peptides is virus serotype specific rather than cross-reactive.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-78-5-1065