Comparative morphometric study of immunohistochemical versus conventional staining for the evaluation of megakaryocytopoiesis in normal and pathological bone marrow biopsies

• Published in: Pathology international 1997-05, Vol.47 (5), p.301-307
• Main Authors: Marisavijević, Dragomir, Radoševič-Radojkovič, Nina, Rolovič, Zoran
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biopsy; Bone Marrow - chemistry; Bone Marrow - pathology; Cell Count; Cell Nucleus - ultrastructure; Cell Size; Evaluation Studies as Topic; Female; Hematopoiesis - physiology; Humans; Immunohistochemistry; Male; Megakaryocytes - physiology; Megakaryocytes - ultrastructure; megakatyocytopoiesis; Middle Aged; morphometry; myelodysplastic syndrome; Reference Values; Staining and Labeling
• ISSN: 1320-5463; 1440-1827
• DOI: 10.1111/j.1440-1827.1997.tb04497.x

ldentification of megakatyocytes by immunohistochemistry may be superior to hematoxylin‐eosin (HE) stain method for assessing megakaryocyte size and number In clinical specimens; however, a sidsby‐side comparison of the two methods has not been reported. In the present study, comparative morphometry using both methods was performed on marrow biopsies of normal individuals, and of patients with myela‐dysplastic syndrome, chronic myeloid leukemia and Immune thrombocytopenia. Morphometrlc results In the present study showed that precise megakaryocyte size can be calculated in normal and pathologic bone marrow sections by using HE stain if one employs stereological corrections. In contrast, megakaryocyte numbers can be more precisely detected by immunohistochemistry than by HE stain, particularly In myelodyspiastic syndrome and chronic myeloid leukemia. Differentiation disturbances and ineffective megakaryocytopolesls In myelodysplastic syndrome were demonstrated by immunomorphometric analyses.