A differential hybridization scheme to identify photoreceptor-specific genes

Identification of genes expressed preferentially or exclusively in photoreceptors will facilitate the understanding of photoreceptor biology as well as provide candidate genes for inherited retinal degenerations. To achieve this goal we performed a differential hybridization screen of 3717 well-isol...

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Bibliographic Details
Published in:Genome research 1997-05, Vol.7 (5), p.513-521
Main Authors: Swanson, D A, Freund, C L, Steel, J M, Xu, S, Ploder, L, McInnes, R R, Valle, D
Format: Article
Language:English
Subjects:
Base Sequence
Blotting, Northern
Cloning, Molecular - methods
Conserved Sequence
DNA Probes
Evolution, Molecular
Fibroblasts - physiology
Genetic Techniques
Humans
In Situ Hybridization - methods
Molecular Sequence Data
Photoreceptor Cells - physiology
Retina - physiology
Retinal Cone Photoreceptor Cells - physiology
Retinal Rod Photoreceptor Cells - physiology
RNA - genetics
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Summary:Identification of genes expressed preferentially or exclusively in photoreceptors will facilitate the understanding of photoreceptor biology as well as provide candidate genes for inherited retinal degenerations. To achieve this goal we performed a differential hybridization screen of 3717 well-isolated phage clones from a human retinal cDNA library. Clones were selected for further study if they hybridized exclusively or strongly preferentially to a probe derived from RNA isolated from the cone-predominant retina of 13-line ground squirrels as compared to a probe derived from human fibroblast RNA. Twenty percent of clones (9/45) identified by this screen were derived from photoreceptor-specific genes and an additional 24.4% (11/45) were from neural-specific genes, demonstrating the utility of this strategy in identifying genes important for retinal biology.
ISSN:1088-9051
1549-5469
DOI:10.1101/gr.7.5.513