Localization and up-regulation of Mucin (MUC2) gene expression in human nasal biopsies of patients with cystic fibrosis

Using digoxigenin–UTP‐labelled human HAM‐1 (92 bp) or SMUC41 (850 bp) cRNA probes, the expression and localization of MUC2 gene transcripts were determined by in situ hybridization in human nasal tissues obtained as biopsies from 12 patients with cystic fibrosis (CF): all had been part of a gene the...

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Bibliographic Details
Published in:The Journal of pathology 1997-03, Vol.181 (3), p.305-310
Main Authors: Li, Dechun, Wang, Danming, Majumdar, Swati, Jany, Berthold, Durham, Stephen R., Cottrell, Janet, Caplen, Natasha, Geddes, Duncan M., Alton, Eric W. F. W., Jeffrey, Peter K.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Complex syndromes
cystic fibrosis
Cystic Fibrosis - metabolism
DNA, Complementary
Humans
In Situ Hybridization
Male
Medical genetics
Medical sciences
Middle Aged
MUC2
mucin gene expression
Mucin-2
Mucins - metabolism
mucus
Nasal Mucosa - metabolism
RNA, Messenger - genetics
Up-Regulation
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Summary:Using digoxigenin–UTP‐labelled human HAM‐1 (92 bp) or SMUC41 (850 bp) cRNA probes, the expression and localization of MUC2 gene transcripts were determined by in situ hybridization in human nasal tissues obtained as biopsies from 12 patients with cystic fibrosis (CF): all had been part of a gene therapy trial in which CFTR cDNA–liposome complexes had been delivered by topical application to eight and liposome alone to four as a placebo control. For comparison, there were nasal tissues taken at surgical resection from four non‐CF subjects and a further four biopsies taken from normal healthy volunteer controls. Both SMUC41 and HAM‐1 probes provided a strong signal. MUC2 mRNA transcripts were present in serous and mucous acini of submucosal glands, ciliated and basal cells of the surface epithelium, and occasional mononuclear inflammatory cells. The percentages (mean±SEM) of serous and mucous acini showing positivity for MUC2 gene expression in the four samples surgically resected from non‐CF subjects were 25·4±5·6 and 26·7±3·3 per cent, respectively. Compared with the non‐CF subjects, the mean percentage of acini showing MUC2 gene expression in the four placebo‐treated CF subjects was significantly higher for serous (80·5±12·7 per cent; P
ISSN:0022-3417
1096-9896
DOI:10.1002/(SICI)1096-9896(199703)181:3<305::AID-PATH774>3.0.CO;2-D