Urea/DTT solubilization of a recombinant Taenia ovis antigen, 45W, expressed as a GST fusion protein results in enhanced protective immune response to the 45W moiety

Vaccination and challenge infection experiments were conducted in sheep using different forms of a recombinant protein (45W) from the cestode parasite Taenia ovis. 45W was expressed in Escherichia coli as a fusion protein with glutathione-S-transferase (45W-GST) and was produced as both soluble prot...

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Bibliographic Details
Published in:Vaccine 1997-04, Vol.15 (5), p.469-472
Main Authors: Rothel, J.S., Wood, P.R., Seow, H.-F., Lightowlers, M.W.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Helminth - biosynthesis
Antigens, Helminth
Applied microbiology
Biological and medical sciences
Dithiothreitol
Fundamental and applied biological sciences. Psychology
Glutathione Transferase - immunology
Linear Models
Male
Microbiology
protein
recombinant
Recombinant Fusion Proteins - immunology
Sheep
Solubility
solubilization
Taenia - immunology
Urea
vaccine
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Synthetic
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Summary:Vaccination and challenge infection experiments were conducted in sheep using different forms of a recombinant protein (45W) from the cestode parasite Taenia ovis. 45W was expressed in Escherichia coli as a fusion protein with glutathione-S-transferase (45W-GST) and was produced as both soluble protein and insoluble inclusion bodies. Vaccination of animals with either the soluble or inclusion body derived protein resulted in the immune response being predominantly directed to the GST moiety of 45W-GST. Conversely, vaccination with 45W-GST which had been solubilizedltreated with urea and dithiothreitol (DTT), elicited enhanced responses to the 45W moiety and significantly reduced responses to GST. Vaccination with all forms of 45W-GST protected sheep against experimental T. ovis infection. However, protection was highly correlated with anti-45W antibody levels and these were significantly higher in animals vaccinated with the urea/DTT treated form of 45W-GST. It is suggested that recombinant proteins expressed either with or without fusion partners may stimulate enhanced immune responses when incorporated in vaccine formulations in a denatured/reduced state.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(96)00229-0