Detergent effects on the light-harvesting chlorophyll A/B-protein complex crystallization revealed by fluorescence depolarization

Detergent effects on the pre-crystallization of light-harvesting chlorophyll a/b-protein complex (LHCII) were investigated through the fluorescence depolarization method. Stable LHCII crystals were formed in the media containing Triton X-100 (TX) or n-nonyl-beta-D-glucopyranoside (NG) and the crysta...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1997-04, Vol.233 (2), p.555-558
Main Authors: Furuichi, M, Nishimoto, E, Koga, T, Takase, A, Yamashita, S
Format: Article
Language:English
Subjects:
Chlorophyll - metabolism
Crystallization
Detergents - pharmacology
Fluorescence Polarization
Light-Harvesting Protein Complexes
Photosynthetic Reaction Center Complex Proteins - drug effects
Spinacia oleracea
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Summary:Detergent effects on the pre-crystallization of light-harvesting chlorophyll a/b-protein complex (LHCII) were investigated through the fluorescence depolarization method. Stable LHCII crystals were formed in the media containing Triton X-100 (TX) or n-nonyl-beta-D-glucopyranoside (NG) and the crystallization efficients were dependent on their concentrations. The second virial coefficient of crystallizing system, estimated by the fluorescence depolarization analysis of LHCII, showed the most harmonious value under the condition yielding the most efficient crystallization of LHCII to suggest that some specific molecular interaction leading to the crystal growth would be induced according to the concentration of TX or NG.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.6429