Cloning and characterization of the citA gene encoding the mitochondrial citrate synthase of Aspergillus nidulans

We isolated a citrate synthase gene (citA) from Aspergillus nidulans. By analysis of the protein coding region, citA was shown to encode a citrate synthase (CitA) of 52.2 kDa consisting of 474 amino acid residues that were interrupted by seven introns. Also, the precursor CitA protein was revealed t...

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Bibliographic Details
Published in:Molecules and cells 1997-04, Vol.7 (2), p.290-295
Main Authors: Park, B.W, Han. K.H, Lee, C.Y, Maeng, P.J. (Chungnam National University, Taejon (Korea Republic). Department of Microbiology), Lee, C.H. (Korea Ginseng & Tobacco Research Institute, Taejon (Korea Republic))
Format: Article
Language:English
Subjects:
ACIDE AMINE
Amino Acid Sequence
AMINO ACIDS
AMINOACIDOS
Animals
ASPERGILLUS NIDULANS
Aspergillus nidulans - enzymology
Aspergillus nidulans - genetics
Base Sequence
CITRATE
Citrate (si)-Synthase - genetics
CITRATES
CITRATOS
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
DNA Primers - genetics
DNA, Fungal - genetics
Genes, Fungal
GENOMAS
GENOME
GENOMES
LIGASAS
LIGASE
LIGASES
Mitochondria - enzymology
MOLECULAR CLONING
Molecular Sequence Data
MOLECULAR WEIGHT
PCR
PESO MOLECULAR
POIDS MOLECULAIRE
Restriction Mapping
Sequence Homology, Amino Acid
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Description
Summary:We isolated a citrate synthase gene (citA) from Aspergillus nidulans. By analysis of the protein coding region, citA was shown to encode a citrate synthase (CitA) of 52.2 kDa consisting of 474 amino acid residues that were interrupted by seven introns. Also, the precursor CitA protein was revealed to have an N-terminal mitochondrial targeting signal of 35 amino acid residues containing an R-3 cleavage motif, R(32)-C-Y decreases S(35), which supports the fact that citA encodes the mitochondrial form of citrate synthase of A. nidulans. Southern blot analysis showed that citA is present as a single copy in the genome.
ISSN:1016-8478
DOI:10.1016/S1016-8478(23)13295-X