Aphidicolin-Induced FRA3B Breakpoints Cluster in Two Distinct Regions

The common fragile site at chromosomal band 3p14.2 (FRA3B) is the most sensitive single site in the human genome to induced chromosomal lesions. This fragile site may predispose chromosome 3p to breakage that is commonly observed in lung, renal, and many other cancers. We previously used aphidicolin...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1997-05, Vol.41 (3), p.485-488
Main Authors: Wang, Liang, Paradee, William, Mullins, Chadwick, Shridhar, Ravi, Rosati, Rita, Wilke, Charles M., Glover, Thomas W., Smith, David I.
Format: Article
Language:English
Subjects:
Aphidicolin - pharmacology
Base Sequence
Biological and medical sciences
Chromosome Fragile Sites
Chromosome Fragility
Chromosome Mapping
Chromosomes, Human, Pair 3 - drug effects
Chromosomes, Human, Pair 3 - genetics
Classical genetics, quantitative genetics, hybrids
DNA - genetics
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
Microsatellite Repeats
Molecular Sequence Data
Neoplasms - genetics
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Summary:The common fragile site at chromosomal band 3p14.2 (FRA3B) is the most sensitive single site in the human genome to induced chromosomal lesions. This fragile site may predispose chromosome 3p to breakage that is commonly observed in lung, renal, and many other cancers. We previously used aphidicolin induction of FRA3B expression in a chromosome 3-only somatic cell hybrid to generate a series of hybrids with breakpoints in the 3p14.2 region. These breakpoints were localized to two distinct clusters, separated by 200 kb, that lie on either side of a region of frequent breakage within FRA3B as observed by FISH analysis. Seven proximal aphidicolin-induced breakpoints were localized at or near the end of a THE element. The THE-1 element is flanked by LINE andAlurepetitive elements. The eight distal aphidicolin-induced breakpoints clustered in a region capable of forming multiple hairpin-like structures. Thus repetitive elements and hairpin-like structures may be responsible for chromosome fragility in this region.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1997.4690