Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids

A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted...

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Bibliographic Details
Published in:Biology of reproduction 1997-06, Vol.56 (6), p.1383-1389
Main Authors: LI, M.-W, YUDIN, A. I, VANDEVOORT, C. A, SABEUR, B. K, PRIMAKOFF, P, OVERSTREET, J. W
Format: Article
Language:English
Subjects:
Acrosome - drug effects
Animals
Biological and medical sciences
Chamomile
Cricetinae
Enzyme Inhibitors - pharmacology
Female
Flavonoids - pharmacology
Fundamental and applied biological sciences. Psychology
Hyaluronoglucosaminidase - antagonists & inhibitors
Hydrolyzable Tannins - pharmacology
In Vitro Techniques
Kaempferols
Macaca fascicularis
Male
Mammalian male genital system
Morphology. Physiology
Oils, Volatile - pharmacology
Plants, Medicinal
Quercetin - analogs & derivatives
Quercetin - pharmacology
Sperm Motility - drug effects
Sperm-Ovum Interactions - drug effects
Spermatozoa - drug effects
Spermatozoa - enzymology
Vertebrates: reproduction
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Summary:A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of 50-200 microM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin, and apigenin at 100 microM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod56.6.1383