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Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids
A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids inhibited the activity of hyaluronidase extracted...
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| Published in: | Biology of reproduction 1997-06, Vol.56 (6), p.1383-1389 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 1389 |
| container_issue | 6 |
| container_start_page | 1383 |
| container_title | Biology of reproduction |
| container_volume | 56 |
| creator | LI, M.-W YUDIN, A. I VANDEVOORT, C. A SABEUR, B. K PRIMAKOFF, P OVERSTREET, J. W |
| description | A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four
flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids
inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of
50-200 microM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin,
and apigenin at 100 microM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a
significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and
their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and
it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not
affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools
for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization. |
| doi_str_mv | 10.1095/biolreprod56.6.1383 |
| format | article |
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flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids
inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of
50-200 microM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin,
and apigenin at 100 microM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a
significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and
their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and
it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not
affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools
for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod56.6.1383</identifier><identifier>PMID: 9166689</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Acrosome - drug effects ; Animals ; Biological and medical sciences ; Chamomile ; Cricetinae ; Enzyme Inhibitors - pharmacology ; Female ; Flavonoids - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hyaluronoglucosaminidase - antagonists & inhibitors ; Hydrolyzable Tannins - pharmacology ; In Vitro Techniques ; Kaempferols ; Macaca fascicularis ; Male ; Mammalian male genital system ; Morphology. Physiology ; Oils, Volatile - pharmacology ; Plants, Medicinal ; Quercetin - analogs & derivatives ; Quercetin - pharmacology ; Sperm Motility - drug effects ; Sperm-Ovum Interactions - drug effects ; Spermatozoa - drug effects ; Spermatozoa - enzymology ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1997-06, Vol.56 (6), p.1383-1389</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2676380$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9166689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LI, M.-W</creatorcontrib><creatorcontrib>YUDIN, A. I</creatorcontrib><creatorcontrib>VANDEVOORT, C. A</creatorcontrib><creatorcontrib>SABEUR, B. K</creatorcontrib><creatorcontrib>PRIMAKOFF, P</creatorcontrib><creatorcontrib>OVERSTREET, J. W</creatorcontrib><title>Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four
flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids
inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of
50-200 microM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin,
and apigenin at 100 microM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a
significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and
their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and
it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not
affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools
for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.</description><subject>Acrosome - drug effects</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chamomile</subject><subject>Cricetinae</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Female</subject><subject>Flavonoids - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hyaluronoglucosaminidase - antagonists & inhibitors</subject><subject>Hydrolyzable Tannins - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Kaempferols</subject><subject>Macaca fascicularis</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Oils, Volatile - pharmacology</subject><subject>Plants, Medicinal</subject><subject>Quercetin - analogs & derivatives</subject><subject>Quercetin - pharmacology</subject><subject>Sperm Motility - drug effects</subject><subject>Sperm-Ovum Interactions - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - enzymology</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpFkVtL7TAQhYMc0e3lFxyEPBx86zaXdto-ingDwRd9LpMmtfGkyTZp3fTfW3WjTwtmLb4Z1hDyl7M1Z3VxoWxw0Wxi0AWsYc1lJffIiheizkoB1R-yYoxBJiXIQ3KU0itjPJdCHpCDmgNAVa-Ivve9VXa0wdPQ0SH4_2amaWPiQPsZ3RSDtxqTodiO9t2OM0WvaW9GE4MLL2FKtJ2GyS26Md6MEb9Yaqadw_fgg9XphOx36JI53ekxeb65frq6yx4eb--vLh-yXgCMWYtYlK2SXHAsqlppNELnHEWNZZ2DEiAUA4Ui1xVIWMaslEqoKmddIYSUx-T8m7t08jaZNDaDTa1xDr1ZDm3KmgngDJbg2S44qcHoZhPtgHFudrUs_r-dj6lF10X0rU0_MQElyIr97uvtS7-10TRpQOcWqGy2220BDTSfX5EfUeKC9g</recordid><startdate>19970601</startdate><enddate>19970601</enddate><creator>LI, M.-W</creator><creator>YUDIN, A. I</creator><creator>VANDEVOORT, C. A</creator><creator>SABEUR, B. K</creator><creator>PRIMAKOFF, P</creator><creator>OVERSTREET, J. W</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19970601</creationdate><title>Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids</title><author>LI, M.-W ; YUDIN, A. I ; VANDEVOORT, C. A ; SABEUR, B. K ; PRIMAKOFF, P ; OVERSTREET, J. W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-caa57cb3121a589bdae2d41a29a7946b262b06ba24d863629a073b2b840f52233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Acrosome - drug effects</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chamomile</topic><topic>Cricetinae</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Female</topic><topic>Flavonoids - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hyaluronoglucosaminidase - antagonists & inhibitors</topic><topic>Hydrolyzable Tannins - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Kaempferols</topic><topic>Macaca fascicularis</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Oils, Volatile - pharmacology</topic><topic>Plants, Medicinal</topic><topic>Quercetin - analogs & derivatives</topic><topic>Quercetin - pharmacology</topic><topic>Sperm Motility - drug effects</topic><topic>Sperm-Ovum Interactions - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - enzymology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LI, M.-W</creatorcontrib><creatorcontrib>YUDIN, A. I</creatorcontrib><creatorcontrib>VANDEVOORT, C. A</creatorcontrib><creatorcontrib>SABEUR, B. K</creatorcontrib><creatorcontrib>PRIMAKOFF, P</creatorcontrib><creatorcontrib>OVERSTREET, J. W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LI, M.-W</au><au>YUDIN, A. I</au><au>VANDEVOORT, C. A</au><au>SABEUR, B. K</au><au>PRIMAKOFF, P</au><au>OVERSTREET, J. W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1997-06-01</date><risdate>1997</risdate><volume>56</volume><issue>6</issue><spage>1383</spage><epage>1389</epage><pages>1383-1389</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>A microplate assay for hyaluronidase and a heterologous cumulus penetration assay were used to determine the effects of four
flavonoids (tannic acid, kaempferol, quercetin, and apigenin) on the function of cynomolgus monkey sperm. All four flavonoids
inhibited the activity of hyaluronidase extracted from monkey sperm in a concentration-dependent manner over the range of
50-200 microM. Tannic acid and apigenin had lower inhibitory effects than kaempferol and quercetin. Kaempferol, quercetin,
and apigenin at 100 microM were shown to significantly inhibit monkey sperm penetration into hamster cumulus. There was a
significant linear relationship between the capacity of the flavonoids to inhibit monkey sperm hyaluronidase activity and
their inhibitory effects on hamster cumulus penetration (r = 0.97). Tannic acid was observed to reduce sperm motility, and
it was not used in the cumulus penetration assay. The other three flavonoids tested in the cumulus penetration assay did not
affect sperm motility, nor did they induce acrosome reactions. The results demonstrate that the flavonoids are useful tools
for assessing the involvement of hyaluronidase in the functions of monkey sperm that are involved in fertilization.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>9166689</pmid><doi>10.1095/biolreprod56.6.1383</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1997-06, Vol.56 (6), p.1383-1389 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79026106 |
| source | Oxford Journals Online |
| subjects | Acrosome - drug effects Animals Biological and medical sciences Chamomile Cricetinae Enzyme Inhibitors - pharmacology Female Flavonoids - pharmacology Fundamental and applied biological sciences. Psychology Hyaluronoglucosaminidase - antagonists & inhibitors Hydrolyzable Tannins - pharmacology In Vitro Techniques Kaempferols Macaca fascicularis Male Mammalian male genital system Morphology. Physiology Oils, Volatile - pharmacology Plants, Medicinal Quercetin - analogs & derivatives Quercetin - pharmacology Sperm Motility - drug effects Sperm-Ovum Interactions - drug effects Spermatozoa - drug effects Spermatozoa - enzymology Vertebrates: reproduction |
| title | Inhibition of monkey sperm hyaluronidase activity and heterologous cumulus penetration by flavonoids |
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