Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in human skin and other basement membranes

A variety of heparan sulfate proteoglycans (HSPG) have been identified on cell surfaces and in basement membrane (BM). To more fully characterize HSPG in human skin BM, we used two monoclonal antibodies (MAb) directed against epitopes of the core protein of a high molecular weight HSPG isolated from...

Full description

Saved in:
Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1989-07, Vol.37 (7), p.961-970
Main Authors: Horiguchi, Y, Couchman, JR, Ljubimov, AV, Yamasaki, H, Fine, JD
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal
Basement Membrane - analysis
Basement Membrane - ultrastructure
Biological and medical sciences
Carbohydrates
Endothelium - analysis
Epidermis - analysis
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Gestational Age
Heparin - analogs & derivatives
Heparin - analysis
Heterosides
Histocytochemistry
Humans
Molecular Weight
Other biological molecules
Proteoglycans - analysis
Skin - analysis
Skin - embryology
Skin - ultrastructure
Tissue Distribution
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A variety of heparan sulfate proteoglycans (HSPG) have been identified on cell surfaces and in basement membrane (BM). To more fully characterize HSPG in human skin BM, we used two monoclonal antibodies (MAb) directed against epitopes of the core protein of a high molecular weight HSPG isolated from murine EHS tumor. Indirect immunofluorescence revealed linear distribution of HSPG within all skin BM, and within BM of all other human organs investigated. In a study of the ontogeny of HSPG in human skin BM, HSPG was detectable as early as 54 gestational days, comparable with other ubiquitous BM components, such as laminin and type IV collagen. Immunoelectron microscopy on adult skin and neonatal foreskin showed staining primarily within the lamina densa (LD) and sub-lamina densa regions of the dermoepidermal junction (DEJ) and vascular BM. In neonatal foreskin, additional staining was noted of basilar cytoplasmic membranes of keratinocytes, endothelial cells, and pericytes. We conclude that the core protein of a high molecular weight HSPG is ubiquitous in human BM, appears in fetal skin on or before 54 days, and is present primarily in the regions of the LD and sub-LD.
ISSN:0022-1554
1551-5044
DOI:10.1177/37.7.2659664