DISCREPANCY BETWEEN LATEX AND ELISA D-DIMER VALUES IN SEPSIS MAY BE CAUSED BY HUMAN NEUTROPHIL ELASTASE

We have recently shown that D-dimers are degraded by human neutrophil elastase (HNE) in vitro, causing rapid decrease in the D-dimer levels measured by a Latex test, but not with an ELISA test employing the same monoclonal antibody against D-dimer. To see if such discrepant D-dimer concentrations oc...

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Bibliographic Details
Published in:Thrombosis research 1997-04, Vol.86 (2), p.141-152
Main Authors: Bach-Gansmo, Edvin T, Strand, Øystein, Godal, Hans Christian, Wisløff, Finn, Skjønsberg, Ole H
Format: Article
Language:English
Subjects:
Adult
alpha 1-Antitrypsin - analysis
Biological and medical sciences
Blood coagulation
Case-Control Studies
D-dimer
Elastase
ELISA test
Enzyme-Linked Immunosorbent Assay - methods
Female
Fibrin Fibrinogen Degradation Products - analysis
Fibrinolysis
Humans
In Vitro Techniques
Investigative techniques, diagnostic techniques (general aspects)
Latex agglutination test
Latex Fixation Tests - methods
Leukocyte Elastase - blood
Male
Medical sciences
Middle Aged
Neutrophils - enzymology
Pre-Eclampsia - blood
Pregnancy
sepsis
Sepsis - blood
Sepsis - enzymology
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Summary:We have recently shown that D-dimers are degraded by human neutrophil elastase (HNE) in vitro, causing rapid decrease in the D-dimer levels measured by a Latex test, but not with an ELISA test employing the same monoclonal antibody against D-dimer. To see if such discrepant D-dimer concentrations occurred in patients with high HNE concentration, we examined 80 plasma samples from 8 patients with sepsis with a Latex and an ELISA test and calculated the ratio between the D-dimer values obtained with the two tests. Twenty healthy pregnant and twenty pre-eclamptic patients, who are known to have raised D-dimer but low HNE concentrations, were chosen as controls. HNE levels were estimated by determining the HNE-alpha 1-proteinase inhibitor complex (HNE-A1PI) concentration. HNE-A1PI concentration was increased in sepsis patients compared with pre-eclamptic patients (p
ISSN:0049-3848
1879-2472
DOI:10.1016/S0049-3848(97)00057-1