Quantitative comparison of mu opioid receptor mRNA in selected CNS regions of alcohol naive rats selectively bred for high and low alcohol drinking

We combined solution hybridization, ribonuclease protection and microdissection techniques to quantitatively compare the anatomical distribution of mu receptor mRNA in discrete brain regions of alcohol naive rats selectively bred for high and low alcohol drinking (HAD and LAD lines, respectively). T...

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Bibliographic Details
Published in:Neuroscience letters 1997-05, Vol.227 (1), p.9-12
Main Authors: Gong, Jianhua, Li, Xuan-Wen, Lai, Zhennan, Froehlich, Janice C, Yu, Lei
Format: Article
Language:English
Subjects:
Alcohol drinking
Alcohol Drinking - metabolism
Alcoholism and acute alcohol poisoning
Animals
Biological and medical sciences
Brain Chemistry - physiology
Gene Expression
Linear Models
Male
Medical sciences
Mu opioid receptor
Nucleic Acid Hybridization
Rats
Receptors, Opioid, mu - genetics
Ribonucleases
RNA, Messenger - analysis
Sensitivity and Specificity
Solution hybridization
Toxicology
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Summary:We combined solution hybridization, ribonuclease protection and microdissection techniques to quantitatively compare the anatomical distribution of mu receptor mRNA in discrete brain regions of alcohol naive rats selectively bred for high and low alcohol drinking (HAD and LAD lines, respectively). The solution hybridization assay is highly sensitive and can detect mu opioid receptor mRNA in a 100-fold linear range from 4 to 421 amol. HAD and LAD rats exhibited a similar level of mu receptor mRNA in all central nervous system (CNS) regions examined except for the inferior colliculus. Our data suggest that the steady-state level of mu receptor mRNA is not associated with genetic differences in alcohol drinking behavior.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(97)00289-9