Determination of morphine and codeine in plasma by HPLC following solid phase extraction

A cheap simple and rapid extraction procedure followed by a UV high performance liquid chromatography (HPLC) assay is described for the simultaneous determination of morphine (M) and codeine (C) in plasma. The method is based on extraction of these opiates from plasma using reversed phase (solid pha...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1997-03, Vol.15 (6), p.759-764
Main Authors: Freiermuth, Michel, Plasse, Jean-Claude
Format: Article
Language:English
Subjects:
Analgesics, Opioid - blood
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid
Codeine - blood
Codeine analysis
General pharmacology
HPLC of opiates
Humans
Linear Models
Medical sciences
Morphine - blood
Morphine analysis
Pharmacology. Drug treatments
Reproducibility of Results
Solid phase extraction
Spectrophotometry, Ultraviolet
Time Factors
UV detection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cheap simple and rapid extraction procedure followed by a UV high performance liquid chromatography (HPLC) assay is described for the simultaneous determination of morphine (M) and codeine (C) in plasma. The method is based on extraction of these opiates from plasma using reversed phase (solid phase) extractions columns followed by HPLC with UV detection at 240 nm. The extraction step provides, respectively, 85 and 80% recovery for M and C. The response of the detection system is linear for both molecules in the studied range from 50 to 750 ng ml −1. No other drugs have been found to interfere with the assay. This method offers a quick, cost effective and reliable procedure for specifically determining M and C, from a small sample volume.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(96)01911-5