Enhancement of cartilage matrix protein synthesis in arthritic cartilage

Objective. To investigate whether the synthesis of cartilage matrix protein (CMP) is enhanced in arthritic cartilage. Methods. The content of CMP in human and pig cartilage was determined by immunoblotting, and CMP‐producing chondrocytes in osteoarthritic (OA) and rheumatoid arthritic (RA) joints we...

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Bibliographic Details
Published in:Arthritis and rheumatism 1997-06, Vol.40 (6), p.1029-1036
Main Authors: Okimura, Akinobu, Okada, Yasunori, Makihira, Seicho, Pan, Haiou, Yu, Li, Tanne, Kazuo, Imai, Kazushi, Yamada, Harumoto, Kawamoto, Takeshi, Noshiro, Mitsuhide, Yan, Weiqun, Kato, Yukio
Format: Article
Language:English
Subjects:
Adult
Aged
Amino Acid Sequence
Animals
Antibody Specificity
Arthritis, Rheumatoid - metabolism
Biological and medical sciences
Cartilage Oligomeric Matrix Protein
Cartilage, Articular - chemistry
Cartilage, Articular - cytology
Cartilage, Articular - metabolism
Cattle
Diseases of the osteoarticular system
Extracellular Matrix Proteins
Female
Glycoproteins - biosynthesis
Glycoproteins - immunology
Glycoproteins - isolation & purification
Humans
Immunoblotting
Immunohistochemistry
Male
Matrilin Proteins
Medical sciences
Middle Aged
Molecular Sequence Data
Osteoarthritis
Swine
Tissue Distribution
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Summary:Objective. To investigate whether the synthesis of cartilage matrix protein (CMP) is enhanced in arthritic cartilage. Methods. The content of CMP in human and pig cartilage was determined by immunoblotting, and CMP‐producing chondrocytes in osteoarthritic (OA) and rheumatoid arthritic (RA) joints were immunostained. Results. CMP was undetectable in the condylar cartilage and disc of pigs, whereas it was abundant in the rib and tracheal cartilage of the same animals. By immunohistochemical analysis, CMP was localized in only a few chondrocytes (5%) in normal human joints, whereas numerous chondrocytes (>60%) were immunostained in RA joints. The number of CMP‐producing cells was also increased in OA cartilage (>40%). Immunoblotting analyses confirmed that the CMP content in the cartilage from OA and RA patients was much higher than that in normal cartilage. Conclusion. These findings demonstrate that articular chondrocytes can synthesize CMP, although it is suppressed under physiologic conditions. The results also suggest that articular chondrocytes express CMP in response to arthritic stimuli.
ISSN:0004-3591
1529-0131
DOI:10.1002/art.1780400606