Enhanced Expression of the Epstein-Barr Virus Latent Membrane Protein by a Recombinant Vaccinia Virus

Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Wilmslow Road, Manchester M20 9BX, U.K. The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone wa...

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Bibliographic Details
Published in:Journal of general virology 1989-05, Vol.70 (5), p.1231-1237
Main Authors: Stewart, James P, Hampson, Ian N, Heinrich, Hans-Werner, Mackett, Michael, Arrand, John R
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - analysis
Antigens, Viral - genetics
Antigens, Viral - immunology
Base Sequence
Biological and medical sciences
cloning
Cloning, Molecular
Cross Reactions
DNA - genetics
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
gene expression
Gene Expression Regulation
genes
Genes, Viral
Genetics
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - immunology
Humans
latent membrane protein
Microbiology
Molecular Sequence Data
Rabbits
Recombination, Genetic
Tumor Cells, Cultured
vaccinia virus
Vaccinia virus - genetics
Vaccinia virus - immunology
Viral Matrix Proteins
Virology
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Summary:Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Wilmslow Road, Manchester M20 9BX, U.K. The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone was characterized by sequencing and then used to make a recombinant vaccinia virus. This virus (VLMP) was shown to express a relatively high level of LMP in an authentic fashion. Antisera raised in rabbits against VLMP were shown to react with B95-8 LMP as well as cross-reacting with a 50K cellular protein. Keywords: EBV, latent membrane protein, vaccinia virus Present address: VEB Friedrich-Loeffler Institut, 2201 Insel Riems, G.D.R. Received 7 November 1988; accepted 11 January 1989.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-70-5-1231