A New Anthracycline Antibiotic, IT-62-B, Converts the Morphology of ras-Transformed Cells Back to Normal: Taxonomy, Fermentation, Isolation, Structure Elucidation and Biological Characterization

A new antibiotic, IT-62-B was isolated from the culture broth of Streptomyces sp. IT-62 by extraction with acetone and then with ethyl acetate, followed by conventional column chromatography using silica gel, Sephadex LH-20 and silica ODS. Its structure (C39H47NO15, MW 769) was determined by 1H, 13C...

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Bibliographic Details
Published in:Journal of antibiotics 1997/04/25, Vol.50(4), pp.297-303
Main Authors: KAWAUCHI, TAKASHI, SASAKI, TORU, YOSHIDA, KEN-ICHIRO, MATSUMOTO, HIROSHI, CHEN, RU-XIAN, HUANG, MING-YU, OTANI, TOSHIO
Format: Article
Language:English
Subjects:
Animals
Anthracyclines
Antibiotics, Antineoplastic - chemistry
Antibiotics, Antineoplastic - isolation & purification
Antibiotics, Antineoplastic - pharmacology
Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents
Antineoplastic agents
Antineoplastic Agents - chemistry
Antineoplastic Agents - isolation & purification
Antineoplastic Agents - pharmacology
Applied microbiology
Biological and medical sciences
Chemotherapic agents
Chemotherapy
Fermentation
Fundamental and applied biological sciences. Psychology
Genes, ras - drug effects
Humans
Leukemia L1210 - drug therapy
Medical sciences
Mice
Microbial Sensitivity Tests
Microbiology
Molecular Structure
Pharmacology. Drug treatments
Streptomyces
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Summary:A new antibiotic, IT-62-B was isolated from the culture broth of Streptomyces sp. IT-62 by extraction with acetone and then with ethyl acetate, followed by conventional column chromatography using silica gel, Sephadex LH-20 and silica ODS. Its structure (C39H47NO15, MW 769) was determined by 1H, 13C NMR, MS, IR and UV spectrometric techniques to be a new member of the baumycin-group anthracyclines. It showed moderate activity against Gram-positive bacteria and had antitumor activity against various tumor cell lines. Further, antibiotic IT-62-B converted the morphology of ras-transformed NIH3T3 cells and T-cells back to normal at concentrations inhibiting cell growth by 30% or more.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.50.297