SNA-8073-B, a New Isotetracenone Antibiotic Inhibits Prolyl Endopeptidase: I. Fermentation, Isolation and Biological Properties

• Published in: Journal of antibiotics 1997/04/25, Vol.50(4), pp.291-296
• Main Authors: KIMURA, KEN-ICHI, KANOU, FUMIKO, KOSHINO, HIROYUKI, URAMOTO, MASAKAZU, YOSHIHAMA, MAKOTO
• Format: Article
• Language: English
• Subjects: Anthraquinones - chemistry; Anthraquinones - isolation & purification; Anthraquinones - pharmacology; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Applied microbiology; Biological and medical sciences; Chromatography, High Pressure Liquid; Fermentation; Fundamental and applied biological sciences. Psychology; General aspects; Medical sciences; Microbiology; Pharmacology. Drug treatments; Serine Endopeptidases - metabolism; Serine Proteinase Inhibitors - chemistry; Serine Proteinase Inhibitors - isolation & purification; Serine Proteinase Inhibitors - pharmacology; Stereoisomerism; Streptomyces
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.50.291

SNA-8073-B, an inhibitor of prolyl endopeptidase isolated from the broth filtrate of Streptomyces sp. SNA-8073, is a new isotetracenone antibiotic. It was purified by ethyl acetate extraction, silica gel column chromatography and high performance liquid chromatography on ODS column. SNA-8073-B has the molecular formula of C20H16O5 and is a stereoisomer of SNA-8073-A (fujianmycin B, rubiginone A2). SNA-8073-B inhibited prolyl endopeptidase of Flavobacterium non-competitively (IC50=8.9μM) when Z-Gly-Pro-pNA was used as a substrate, but SNA-8073-A did not show any inhibition even at 60μM.