Fast specific separation and sensitive quantification of bactericidal and sporicidal aldehydes by high-performance liquid chromatography: example of glutaraldehyde determination

• Published in: Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-04, Vol.692 (1), p.79-86
• Main Authors: Menet, M.-C., Gueylard, D., Fievet, M.-H., Thuillier, A.
• Format: Article
• Language: English
• Subjects: Aldehydes; Biological and medical sciences; Chromatography, Gas; Chromatography, High Pressure Liquid; Disinfectants - analysis; Drug Stability; Glutaral - analysis; Glutaraldehyde; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Phenylhydrazines; Reproducibility of Results; Sensitivity and Specificity
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(96)00455-0

This article describes the design and the validation of the HPLC determination of glutaraldehyde at g/l and mg/l concentrations, after derivatization by 2,4-dinitrophenylhydrazine and using the external standard method. At low concentrations, the reaction mixture needs to be heated and a weight ratio of 500 for the 2,4-dinitrophenylhydrazine reagent and the glutaraldehyde ensures a linear calibration curve. In contrast, high concentrations do not require heating of the reaction mixture and a weight ratio of 32 proved to be sufficient. The optimized HPLC method has been validated for both ranges of concentrations. Between 1.25 and 10 mg/l, the content can be determined by the external standard method, with a repeatability of 0.5%. The detection limit is 0.2 mg/l. Between 0.31 and 2.5 g/l, the content can also be determined by the external standard method, with a repeatability of 0.4%. Finally, statistical analysis has demonstrated that aqueous solutions of glutaraldehyde are stable for at least three days at 4°C within the mg to g range.