An anti- χ-1 antibody recognizes a heavily glycosylated protein in rat brain

The χ-1 subunit is a recently identified member of a new class of the ionotropic glutamate receptor family that attenuates NMDA receptor current. We have generated a polyclonal C-terminal antibody to the χ-1 subunit which recognizes a 135-kDa protein in membranes prepared from χ-1 transfected HEK-29...

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Bibliographic Details
Published in:Brain research. Molecular brain research. 1997-06, Vol.46 (1), p.85-90
Main Authors: Ciabarra, Anthony M, Sevarino, Kevin A
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Antibody
Blotting, Western
Brain - metabolism
Brain Chemistry
Cell Line - metabolism
Glutamate
Glycosylation
Hybridization, in situ
mRNA
Nerve Tissue Proteins - analysis
Nerve Tissue Proteins - metabolism
NMDA
Rat brain
Rats
Receptor
Receptors, N-Methyl-D-Aspartate - immunology
Receptors, N-Methyl-D-Aspartate - metabolism
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Summary:The χ-1 subunit is a recently identified member of a new class of the ionotropic glutamate receptor family that attenuates NMDA receptor current. We have generated a polyclonal C-terminal antibody to the χ-1 subunit which recognizes a 135-kDa protein in membranes prepared from χ-1 transfected HEK-293 cells and in rat brain. In the post-natal day 7 (P7) rat brain, Western blot analysis revealed a 135-kDa band in the thalamus and cortex but not the striatum, cerebellum or peripheral tissues. De-glycosylation of the χ-1 subunit in both transfected cell lines and in the brain reduced the 135-kDa band to 110 kDa, near the predicted molecular weight of the χ-1 subunit. These studies demonstrate the χ-1 subunit is expressed as a glycosylated protein subunit in a distribution that parallels that observed for χ-1 mRNA by in situ hybridization.
ISSN:0169-328X
1872-6941
DOI:10.1016/S0169-328X(96)00275-6